Canine CXCL1 ELISA试剂盒(GRO alpha) (ab273183)
Key features and details
- Sensitivity: 5.4 pg/ml
- Range: 5.47 pg/ml - 350 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
概述
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产品名称
Canine CXCL1 ELISA试剂盒(GRO alpha)
参阅全部 CXCL1/GRO alpha 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
5.4 pg/ml -
范围
5.47 pg/ml - 350 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 134.8 121% - 145% Plasma 128.3 109% - 141% Cell culture media 128.8 108% - 149% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Dog -
产品概述
Canine CXCL1 ELISA Kit (GRO alpha) (ab273183) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine CXCL1 (GRO alpha) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine CXCL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Canine CXCL1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine CXCL1 Antibody 2 vials Canine CXCL1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
细胞定位
Secreted. - Information by UniProt
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别名
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273183于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Canine CXCL1 ELISA Kit (GRO alpha) standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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