AB273188

Bovine Insulin ELISA试剂盒

Name: Bovine Insulin ELISA试剂盒 (ab273188)| Abcam中文官网
Brand: Abcam Limited
SKU: AB273188
Price: 8552 CNY
Availability: InStock

Bovine Insulin ELISA Kit

Be the first to review this product! Submit a review

(3 Publications)

Bovine Insulin ELISA Kit is a Sandwich ELISA for the measurement of Bovine Insulin in in Biofluids, Cell Culture Media samples from a wide range of species.

查看别名

Insulin, INS

1 Images

Sandwich ELISA - Bovine Insulin ELISA Kit (AB273188)

sELISA

Supplier Data

Sandwich ELISA - Bovine Insulin ELISA Kit (AB273188)

This standard curve is for demonstration only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Mouse, Rat, Cow, Human, Pig

检测类型

Sandwich

灵敏度

= 4.5 µlU/mL

范围

4.5 - 300 µlU/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

产品详情

Bovine Insulin ELISA Kit (ab273188) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine Insulin in serum (Bovine Insulin concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Bovine Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style

left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

style

left-column

回收率

[ { "sample": "Serum", "range": "72 - 114 %", "average": "= 90.59" }, { "sample": "Plasma", "range": "81 - 122 %", "average": "= 101.9" }, { "sample": "Cell culture media", "range": "87 - 140 %", "average": "= 113.1" } ]

style

left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273188-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273188-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Bovine Insulin coated Microplate (12 x 8 wells)", "number":"AB273188-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273188-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB273188-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273188-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273188-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Bovine Insulin Standard (Lyophilized)", "number":"AB273188-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB273188-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Bovine Insulin Antibody", "number":"AB273188-CMP09", "productcode":"" } ] } } }

style

left-column

性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.

Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.

style

left-column

产品实验方案

style

left-column

靶点信息

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

See full target information INS

style

left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 17:29 PubMed38302986

2024

Bushenhuoluo Decoction improves polycystic ovary syndrome by regulating exosomal miR-30a-5p/ SOCS3/mTOR/NLRP3 signaling-mediated autophagy and pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Huang,Yuanbin Li,Zhuang Chen,Huiping Ou,Yanjiao Tan,Hui Lin

Advances in pharmacological and pharmaceutical sciences 2022:3776676 PubMed35445196

2022

Effects of the Antiobesity Drugs Aplex and Venera on Certain Biochemical and Physiological Indices in Obese Adult Male Albino Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Suzan S A Elpasty,Eman G E Helal,Ashraf M M Algendy,Hany N Yousef

Journal of dairy science 105:4520-4533 PubMed35248377

2022

Overactivation of hepatic mechanistic target of rapamycin kinase complex 1 (mTORC1) is associated with low transcriptional activity of transcription factor EB and lysosomal dysfunction in dairy cows with clinical ketosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyuan Fang,Xinwei Li,Shu Wang,Qianming Jiang,Juan J Loor,Xiuhuan Jiang,Lingxue Ju,Hao Yu,Taiyu Shen,Men Chen,Yuxiang Song,Zhe Wang,Xiliang Du,Guowen Liu

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Bovine Insulin ELISA试剂盒

Bovine Insulin ELISA Kit

Sandwich ELISA - Bovine Insulin ELISA Kit (AB273188)