Bovine IL-13 ELISA试剂盒(ab272466)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.4 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture media, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
概述
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产品名称
Bovine IL-13 ELISA试剂盒
参阅全部 IL-13 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 4.1% 批次间 样品 n Mean SD CV% Serum 3 1.8% -
样品类型
Serum, Cell culture media, Hep Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
3.4 pg/ml -
范围
15.6 pg/ml - 1000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 97 96% - 98% Cell culture media 95 93% - 97% Hep Plasma 77 76% - 79% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Cow -
产品概述
Bovine IL-13 ELISA kit (ab272466) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Bovine IL-13 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neurofilament H with 3.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Bovine IL-13 Capture Antibody 1 x 600µl 10X Bovine IL-13 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Bovine IL-13 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
疾病相关
Allergic rhinitis -
序列相似性
Belongs to the IL-4/IL-13 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Allergic rhinitis
- ALRH
- BHR 1
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272466于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Interpolated concentrations of spike IL-13 in bovine serum, plasma and cell culture supernatant samples.
The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%, cell culture media (RPMI 1640 containing 10% fetal bovine serum) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Example of bovine IL-13 standard curve in Sample Diluent NS.The IL-13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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SimpleStep ELISA Protocol DiagramSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Sandwich ELISA - Bovine IL-13 ELISA Kit (ab272466)To learn more about the advantages of recombinant antibodies see here.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272466 尚未被引用在任何文献中。