Bovine IgG ELISA试剂盒- 45 minutes (ab273152)
Key features and details
- Range: 10 ng/ml - 2000 ng/ml
- Detection method: Colorimetric
- Assay type: Quantitative
概述
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产品名称
Bovine IgG ELISA试剂盒- 45 minutes
参阅全部 IgG 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1/100 32 38µg/ml 6.5 % 1/200 32 44.17µg/ml 7.6 % 1/400 32 41.55µg/ml 10.8 % 批次间 样品 n Mean SD CV% A 1/16 14 7.3µg/ml 5.87 % B 1/128 14 7.7µg/ml 5.89 % Bovine 1/100 16 1.2µg/ml 4.3 % -
检测类型
Quantitative -
范围
10 ng/ml - 2000 ng/ml -
实验步骤
One step assay -
产品概述
The Bovine Immunoglobulin G ELISA Kit (ab273152) provides a rapid and easy method (one step ELISA) for the quantitative determination of bovine IgGs in cell culture supernatants and serums (CS, FCS, NBCS...) and contaminating bovine IgGs in batches of purified antibodies produced in vitro. The kit includes ready-to-use reagents necessary to analyze 89 samples in less than 45 minutes.
A polyclonal antibody specific to bovine IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and bovine IgG present in the sample are bound by the capture antibody. Then, an HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 10 x 96 tests 1 x 96 tests Bovine IgG standard 0.0 ng/ml 1 x 1ml 1 x 0.3ml Bovine IgG standard 1000 ng/ml 1 x 1ml 1 x 0.3ml Bovine IgG standard 125 ng/ml 1 x 1ml 1 x 0.3ml Bovine IgG standard 2000 ng/ml 1 x 1ml 1 x 0.3ml Bovine IgG standard 250 ng/ml 1 x 1ml 1 x 0.3ml Bovine IgG standard 31 ng/ml 1 x 1ml 1 x 0.3ml Bovine IgG standard 500 ng/ml 1 x 1ml 1 x 0.3ml Detection antibody 1 x 120ml 1 x 12ml Pre-coated microwells strips 10 x 96 tests 1 x 96 tests Sample Diluent 1 x 500ml 1 x 30ml Stop solution 1 x 120ml 1 x 12ml TMB Substrate 1 x 120ml 1 x 12ml -
研究领域
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细胞定位
Secreted -
别名
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273152于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
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Bovine Immunoglobulin G Quantification Kit Standard curve
Example standard curve.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab273152 被引用在 1 文献中.
- Raza A et al. Application of quantitative proteomics to discover biomarkers for tick resistance in cattle. Front Immunol 14:1091066 (2023). PubMed: 36793724