Bovine IGF1 ELISA试剂盒(ab273193)
Key features and details
- Sensitivity: 1.23 ng/ml
- Range: 1.23 ng/ml - 300 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
概述
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产品名称
Bovine IGF1 ELISA试剂盒
参阅全部 IGF1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1.23 ng/ml -
范围
1.23 ng/ml - 300 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 108.5 86% - 137% Plasma 97.27 72% - 119% Cell culture media 96.52 79% - 117% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Cow -
产品概述
Bovine IGF-1 ELISA Kit (ab273193) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine IGF-1 in serum (this kit only can detect a free form of bovine IGF1. The free bovine IGF concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Bovine IGF-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine IGF-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 550X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Anti-Bovine IGF1 coated Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated Anti-Bovine IGF1 Antibody 2 vials Bovine IGF1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
研究领域
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功能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
疾病相关
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
序列相似性
Belongs to the insulin family. -
细胞定位
Secreted. - Information by UniProt
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别名
- IBP1
- IGF I
- IGF IA
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273193于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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