Anti-Borrelia burgdorferi IgG ELISA试剂盒(ab178635)
Key features and details
- Sensitivity: 98.6 %
- Sample type: Cit plasma, Hep Plasma, Other biological fluids, Serum
- Detection method: Colorimetric
- Assay type: Indirect
概述
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产品名称
Anti-Borrelia burgdorferi IgG ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Pos serum 7 4.6% 批次间 样品 n Mean SD CV% Pos serum 3 4% -
样品类型
Serum, Other biological fluids, Hep Plasma, Cit plasma -
检测类型
Indirect -
灵敏度
98.6 % -
实验步骤
Multiple steps standard assay -
产品概述
Abcam’s anti- Borrelia burgdorferi IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF.
A 96-well plate has been precoated with Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgG sample captured in plate.
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经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Borrelia burgdorferi (IgG) Coated Microplate (12 x 8 wells) 1 unit Borrelia burgdorferi Anti-IgG HRP Conjugate 1 x 20ml Borrelia burgdorferi Cut-off Control 1 x 3ml Borrelia burgdorferi Negative Control 1 x 2ml Borrelia burgdorferi Positive Control 1 x 2ml Cover Foil 1 unit IgG Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究领域
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相关性
Borrelia burgdorferi is a bacterial species of the spirochete class of the genus Borrelia, which has a diderm (double-membrane) envelope. B. burgdorferi is predominant in North America, but also exists in Europe, and is the agent of Lyme disease. Borrelia burgdorferi is gram negative only by default because safranin is the last dye used, but in reality spirochetes are not classified as either gram positive or negative.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178635于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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