Androstenedione ELISA试剂盒(ab108672)
Key features and details
- Sensitivity: 0.01 ng/ml
- Range: 0.1 ng/ml - 10 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
概述
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产品名称
Androstenedione ELISA试剂盒
参阅全部 Androstenedione 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Control Sera 60 <=10% 批次间 样品 n Mean SD CV% Control Sera 30 <=9.5% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Competitive -
灵敏度
= 0.01 ng/ml -
范围
0.1 ng/ml - 10 ng/ml -
回收率
100.91 %
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实验步骤
Multiple steps standard assay -
产品概述
Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in serum and plasma.
A 96-well plate has been precoated with anti-Androstenedione IgG. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Washing Solution 1 x 50ml Androstenedione Control 1 x 1ml Androstenedione Standard 0 – 0 ng/mL 1 x 1ml Androstenedione Standard 1 – 0.1 ng/mL 1 x 1ml Androstenedione Standard 2 – 0.4 ng/mL 1 x 1ml Androstenedione Standard 3 – 1.2 ng/mL 1 x 1ml Androstenedione Standard 4 – 4.0 ng/mL 1 x 1ml Androstenedione Standard 5 – 10.0 ng/mL 1 x 1ml Androstenedione-HRP Conjugate 1 x 21ml Anti-Androstenedione IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究领域
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别名
- 4 androstenedione
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108672于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108672
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab108672 被引用在 5 文献中.
- Estienne A et al. Chronic dietary exposure to a glyphosate-based herbicide results in reversible increase early embryo mortality in chicken. Ecotoxicol Environ Saf 241:113741 (2022). PubMed: 35679729
- Siroosbakht S et al. Are androstenedione, dihydrotestosterone, thyroid-stimulating hormone, insulin-like growth factor I, and insulin-like growth factor binding protein 3 necessary for isolated micropenis healthy boys' evaluation without any phenotypic abnormalities? A cross-sectional study. Andrologia 54:e14617 (2022). PubMed: 36257721
- Bleach R et al. Steroid Ligands, the Forgotten Triggers of Nuclear Receptor Action; Implications for Acquired Resistance to Endocrine Therapy. Clin Cancer Res 27:3980-3989 (2021). PubMed: 34016642
- Shah AB et al. Elevated androstenedione in young adult but not early adolescent prenatally androgenized female rats. PLoS One 13:e0196862 (2018). PubMed: 29723293
- Ronis MJ et al. EB 2017 Article: Soy protein isolate feeding does not result in reproductive toxicity in the pre-pubertal rat testis. Exp Biol Med (Maywood) 243:695-707 (2018). PubMed: 29763383