AKT1 + AKT2 + AKT3 (pS473) ELISA试剂盒(ab126432)
Key features and details
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Mouse, Rat, Human
概述
-
产品名称
AKT1 + AKT2 + AKT3 (pS473) ELISA试剂盒
参阅全部 AKT1 + AKT2 + AKT3 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell Lysate -
检测类型
Semi-quantitative -
检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse, Rat, Human -
产品概述
ab126432 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated Akt protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. (UniprotID: P31749)
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-Akt (Ser473). An anti-pan Akt antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Akt present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Akt (Ser473) antibody is used to detect phosphorylated Akt (Ser473). After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Akt (Ser473) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with AKT 1/2/3 (pS473) ELISA Kit (ab176635) from our SimpleStep ELISA® range.
-
说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml Akt Microplate (12 strips x 8 wells) coated with anti-pan Akt antibody 1 unit Detection Antibody Akt (Ser473): rabbit anti-phospho-Akt (Ser473) 2 vials 500X HRP-conjugated anti-rabbit IgG 1 x 25µl Positive Control: lyophilized NIH3T3 cell lysate 1 vial Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
-
功能
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
组织特异性
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
结构域
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
翻译后修饰
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
细胞定位
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
-
别名
- AKT
- AKT1
- AKT1 kinase
see all -
数据库链接
- Entrez Gene: 208 Human
- Entrez Gene: 207 Human
- Entrez Gene: 10000 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 11652 Mouse
- Entrez Gene: 23797 Mouse
- Entrez Gene: 24185 Rat
- Entrez Gene: 25233 Rat
see all
相关产品
-
SimpleStep ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126432于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
-
Sandwich ELISA - Akt (pSer473) ELISA Kit (ab126432)The NIH3T3 cells were treated with recombinant human PDGF for 10 minutes to induce phosphorylation of Akt. Serial dilutions of lysates were analyzed in this ELISA.
-
Western blot - Akt (pSer473) ELISA Kit (ab126432)NIH3T3 cells were treated or untreated with recombinant human PDGF for 10 min. Cell lysates were analyzed by Western Blot. -
Sandwich ELISA - Akt (pSer473) ELISA Kit (ab126432)NIH3T3 cells were treated or untreated with recombinant human PDGF for 10 min. Cell lysates were analyzed using this phosphoELISA. -
Sandwich ELISA - Akt (pSer473) ELISA Kit (ab126432)A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab126432 被引用在 2 文献中.
- Luo Y et al. Overexpression of SAPCD2 correlates with proliferation and invasion of colorectal carcinoma cells. Cancer Cell Int 20:43 (2020). PubMed: 32055236
- Aliyu NO et al. Lophirones B and C halt acetaminophen hepatotoxicity by upregulating redox transcription factor Nrf-2 through Akt, PI3K, and PKC pathways. J Biochem Mol Toxicol 32:e22055 (2018). PubMed: 29697884