8 isoprostane ELISA试剂盒(ab175819)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 0.005 ng/ml - 5 ng/ml
- Sample type: Cell culture extracts, Plasma, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Competitive
概述
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产品名称
8 isoprostane ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 12 0.43% 批次间 样品 n Mean SD CV% Overall 12 1.75% -
样品类型
Urine, Serum, Plasma, Cell culture extracts, Tissue -
检测类型
Competitive -
灵敏度
1 pg/ml -
范围
0.005 ng/ml - 5 ng/ml -
实验步骤
Multiple steps standard assay -
产品概述
Abcam’s 8 isoprostane competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the determination of 8 isoprostane levels in biological samples. The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissues phospholipids by oxygen radicals. A recent NIH-sponsored study on Biomarkers of Oxidative Stress has indicated that 8 isoprostane is the best index of oxidative injury in a well-accepted oxidant stress rat model. In addition, plasma 8 isoprostane levels were found to be elevated in elderly subjects with severe hypertension and in urine from subjects with high fat diet-induced liver steatosis.
This kit can be used for the determination of 8 isoprostane in diluted urine, serum, plasma, cells and tissues following proper isolation and purification of the eicosanoid from the isoprostane-containing sample.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 1,000X 8-isoprostane HRP Conjugates 1 x 12µl 10X Sample Dilution Buffer 1 x 25ml 10X Wash Buffer Solution 1 x 25ml 8-isoprotane Standard 1 x 2µl HRP Buffer 1 x 15ml Pre-coated 96-Well Microplate 1 unit TMB Substrate 1 x 22ml -
研究领域
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别名
- Prostaglandin, iso Prostaglandin F2, iso Prostaglandin, iPF2 alpha
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175819于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Example of standard curve obtained with ab175819.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab175819 被引用在 23 文献中.
- García-Sánchez A et al. Prevalence of Hypertension and Obesity: Profile of Mitochondrial Function and Markers of Inflammation and Oxidative Stress. Antioxidants (Basel) 12:N/A (2023). PubMed: 36671026
- Sultan A et al. Oridonin supplementation in rabbits may help to improve the outcome of induced atherosclerosis via autophagy induction. Acta Biomed 94:e2023015 (2023). PubMed: 37092629
- Moufarrej L et al. Oxidative stress response in pulmonary cells exposed to different fractions of PM2.5-0.3 from urban, traffic and industrial sites. Environ Res 216:114572 (2023). PubMed: 36244444
- Evke S et al. Epitranscriptomic Reprogramming Is Required to Prevent Stress and Damage from Acetaminophen. Genes (Basel) 13:N/A (2022). PubMed: 35327975
- Abu Helal R et al. Aortic Fibrosis in Insulin-Sensitive Mice with Endothelial Cell-Specific Deletion of Ceacam1 Gene. Int J Mol Sci 23:N/A (2022). PubMed: 35457157