6-keto-PGF1 alpha ELISA试剂盒(ab133023)
Key features and details
- Sensitivity: 1.4 pg/ml
- Range: 3.2 pg/ml - 50000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human, a wide range of other species
概述
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产品名称
6-keto-PGF1 alpha ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Low conc. 10 41pg/ml 13.1% Medium conc. 10 240pg/ml 4.8% High conc. 10 4886pg/ml 2.9% 批次间 样品 n Mean SD CV% Low conc. 8 239pg/ml 7.9% Medium conc. 8 1220pg/ml 5.2% High conc. 8 4828pg/ml 6% -
样品类型
Cell culture supernatant, Saliva, Urine, Serum, Plasma, Tissue Culture Media -
检测类型
Competitive -
灵敏度
1.4 pg/ml -
范围
3.2 pg/ml - 50000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Saliva 110.7 % - % Urine 108.7 % - % Serum 108.4 % - % Tissue Culture Media 106.7 % - % -
检测时间
3h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human, a wide range of other species -
产品概述
Abcam’s 6-keto-PGF1 alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 6-keto-PGF1 alpha in serum, urine, tissue culture media and other biological fluids.
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-6-keto-PGF1 alpha antigen and a polyclonal sheep antibody specific to 6-keto-PGF1 alpha. After incubation the excess reagents are washed away. pNpp substrate is added. After a short incubation the enzyme reaction is stopped and the yellow color generated by alkaline phosphatase is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 6-keto-PGF1α captured in the plate.
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说明
6-keto-PGF1α is a stable hydrolysis product of prostacyclin (PGI2). Due to the short half-life of PGI2 in buffer (2-3 minutes) the production of prostacyclin is typically monitored by measurement of the 6-keto-PGF1α metabolite and its derivative in urine, 2,3-dinor-6-keto-PGF1α. PGI2 has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia.
Cross Reactivity
Compound Cross Reactivity 6-keto-PGF1α
100% 2,3-dinor-6-keto-PGF1α
3.17% PGF2α 1.67% PGD2 0.60% PGF1α 0.60% PGE1 0.20% 6,15-diketo-13,14-dihydro-PGF1α 0.12% 13,14-dihydro-15-keto-PGF1α <0.01% 15-keto-PGF2α <0.01% PGA2 <0.01% PGB1 <0.01% PGE2 <0.01% Thromboxane B2 <0.01% 15-HpETE <0.01% 2-Arachidonoylglycerol <0.01% Anandamide <0.01% -
经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml 6-keto-PGF1 alpha Alkaline Phosphatase Conjugate 1 x 5ml 6-keto-PGF1 alpha EIA Antibody 1 x 5ml 6-keto-PGF1 alpha Standard 1 x 500µl Assay Buffer 1 x 27ml Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
研究领域
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别名
- 6-keto Prostaglandin F1a
- 6-keto-PGF1a
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133023于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Competitive binding assay - 133023 6-keto-PGF1 alpha ELISA Kit
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Typical Standard CurveRepresentative Standard Curve using ab133023.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab133023 被引用在 2 文献中.
- Yin B et al. The antithrombosis effect of dehydroandrographolide succinate: in vitro and in vivo studies. Pharm Biol 60:175-184 (2022). PubMed: 35014931
- Wang F et al. Antithrombin III/SerpinC1 insufficiency exacerbates renal ischemia/reperfusion injury. Kidney Int 88:796-803 (2015). PubMed: 26108065