24(S)-Hydroxycholesterol ELISA试剂盒(ab204530)
Key features and details
- Sensitivity: 0.78 ng/ml
- Range: 0.39 ng/ml - 100 ng/ml
- Sample type: Cerebral Spinal Fluid, Tissue Culture Media, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Competitive
概述
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产品名称
24(S)-Hydroxycholesterol ELISA试剂盒
参阅全部 Cholesterol 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Buffer 1 24 28.2ng/ml = 9.3% Buffer 2 24 9ng/ml = 5.8% 批次间 样品 n Mean SD CV% Buffer 1 10 28.6ng/ml = 18.4% Buffer 2 10 11.1ng/ml = 18.5% -
样品类型
Tissue Culture Media, Tissue Homogenate, Cerebral Spinal Fluid -
检测类型
Competitive -
灵敏度
= 0.78 ng/ml -
范围
0.39 ng/ml - 100 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Tissue Culture Media = 101.7 % - % Tissue Homogenate = 98.6 % - % Cerebral Spinal Fluid = 99 % - % -
检测时间
2h 00m -
实验步骤
Multiple steps standard assay -
产品概述
Abcam’s 24(S)-Hydroxycholesterol (24-OHC) kit (ab204530) is an in-vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of 24-OHC levels in tissue culture media, cerebral spinal fluid and tissue homogenate samples.
The homeostasis and trafficking of cholesterol is an essential component of both the central and peripheral nervous system in the maintenance of neuronal tissues. Disturbances in this homeostasis may be due to the onset of various neurological diseases such as Alzheimer’s Disease, Huntington’s Disease and multiple sclerosis.
Apolipoprotein E and Cyp46 (also known as 24S-Cholesterol Hydroxylase) are both important in the homeostasis of cerebral cholesterol 6 and thus are of clinical interest in understanding the relation of these molecules with the pathogenesis of these, and potentially other, neurodegenerative diseases.
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说明
24-OHC, an enzymatically-generated side chain-hydroxylated derivative of cholesterol, is a pivotal marker in the study of cerebral cholesterol homeostasis. Cholesterol is unable to cross the blood-brain barrier however, Cyp46 enzyme converts cholesterol to the more soluble 24-OHC, and this hydroxylated form of cholesterol is able to cross the bloodbrain barrier.7,8 This conversion allows for the reduction of cholesterol in the brain and the efflux of 24-OHC from the brain into cerebral spinal fluid and blood. The flux of 24-OHC has been observed in patients with a variety of neurodegenerative diseases. In the instance of Alzheimer’s disease, the change in 24S-hydroxycholesterol concentrations may be indicative of different pathogenetic mechanisms and/or the progression of the disease.3 As in the case of multiple sclerosis, concentrations of 24-OHC have been shown to decrease, likely due to the loss of neuronal cells responsible for the synthesis.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 24(S)-Hydroxycholesterol Antibody (1X Soln.) 1 x 5ml 24(S)-Hydroxycholesterol Conjugate (100X Conc.) 1 x 70µl 24(S)-Hydroxycholesterol Standard 1 x 70µl Assay Buffer 40 1 x 50ml Goat anti-Rabbit IgG coated microplate (12x 8 well strips) 1 unit Plate Sealer 3 units Stop Solution (1N Soln.) 1 x 10ml Streptavidin-HRP (1X Soln.) 1 x 20ml TMB Substrate 2 x 10ml Wash Buffer Concentrate (20X Conc.) 1 x 27ml -
研究领域
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相关性
Cholesterol is a waxy substance that occurs naturally in all parts of the body and that the body needs to function normally. It is present in cell walls or membranes everywhere in the body, including the brain, nerves, muscle, skin, liver, intestines, and heart. The body uses cholesterol to produce many hormones, vitamin D, and the bile acids that help to digest fat. It takes only a small amount of cholesterol in the blood to meet these needs. However, if you have too much cholesterol in your bloodstream, it can lead to atherosclerosis, a condition in which fat and cholesterol are deposited in the walls of the arteries in many parts of the body, including the coronary arteries feeding the heart. In time, narrowing of the coronary arteries by atherosclerosis can produce the signs and symptoms of heart disease, including angina and heart attack.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab204530于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Dose Responce Curve
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Standard Curve
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab204530 被引用在 4 文献中.
- Hosseini E et al. Maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring. PLoS One 17:e0273206 (2022). PubMed: 35998127
- Lu F et al. Serum 24S-hydroxycholesterol predicts long-term brain structural and functional outcomes after hypoxia-ischemia in neonatal mice. J Cereb Blood Flow Metab 41:312-323 (2021). PubMed: 32169014
- Mitroi DN et al. NPC1 enables cholesterol mobilization during long-term potentiation that can be restored in Niemann-Pick disease type C by CYP46A1 activation. EMBO Rep 20:e48143 (2019). PubMed: 31535451
- Lu F et al. Upregulation of cholesterol 24-hydroxylase following hypoxia-ischemia in neonatal mouse brain. Pediatr Res 83:1218-1227 (2018). PubMed: 29718007