20 HETE ELISA试剂盒(ab175817)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 0.01 ng/ml - 1000 ng/ml
- Sample type: Cell culture extracts, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Competitive
概述
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产品名称
20 HETE ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 12 = 0.3% 批次间 样品 n Mean SD CV% Overall 12 = 0.78% -
样品类型
Serum, Plasma, Cell culture extracts, Tissue -
检测类型
Competitive -
灵敏度
= 10 pg/ml -
范围
0.01 ng/ml - 1000 ng/ml -
实验步骤
Multiple steps standard assay -
产品概述
Abcam’s 20 HETE competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the determination of 20 HETE (also known as 20-OH-AA) levels in biological samples. The specificity of the 20 HETE ELISA was investigated using authentic 20 HETE and a panel of fatty acids which, based on their structure, might be anticipated to compete with 20 HETE for binding to antibodies for 20 HETE. Anti-20 HETE did not cross-react with 14,15- and 11,12-DHETs, PGE2 and showed almost no cross-reactivity even with structurally extremely similar arachidonic acid (AA), linoleic acid and linolenic acid as shown in the competitive ELISA analysis. Considering the only difference between 20-HETE and AA is an oxygen molecule, the specificity of this kit is a surprise.
Human essential and salt-sensitive hypertensions were related to differential AA metabolism by cytochrome P450 (CYP) 4A which has AA-ω-hydroxylase (20 HETE synthesis) activity. Increased circulating insulin inhibits 20 HETE synthesis in obese hypertensive subjects.
Recently, CYP4F2 genetic variants, which increased urinary 20 HETE secretion, were found to be correlated with the risk for hypertension in a Chinese population.
This kit can be used for the determination of 20 HETE in serum, plasma, cells and tissues following proper isolation and purification.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 1000X 20-HETE HRP Conjugates 1 x 12µl 10X Sample Dilution Buffer 1 x 25ml 10X Wash Buffer Solution 1 x 25ml 20-HETE Standard 1 x 2µl HRP Buffer 1 x 15ml Pre-Coated 96-Well Microplate 1 unit TMB Substrate 1 x 22ml -
研究领域
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别名
- cytochrome P450,20-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175817于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Example of standard curve obtained with ab175817.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab175817 被引用在 2 文献中.
- Cui W et al. 20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1a pathway: A translational study. Cell Prolif 54:e12964 (2021). PubMed: 33314534
- Li H et al. Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction through AA Metabolism Modulated CYPs Pathway. Int J Biol Sci 15:2037-2050 (2019). PubMed: 31592228