17 beta Estradiol ELISA试剂盒(ab108667)
Key features and details
- Sensitivity: 8.68 pg/ml
- Range: 20 pg/ml - 2000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mammals
概述
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产品名称
17 beta Estradiol ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 20 <=9% 批次间 样品 n Mean SD CV% Overall 3 <=10% -
样品类型
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Competitive -
灵敏度
8.68 pg/ml -
范围
20 pg/ml - 2000 pg/ml -
回收率
101.09 %
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实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mammals -
产品概述
Abcam’s 17 beta Estradiol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 beta Estradiol in serum and plasma (citrate).
A 96-well plate has been precoated with anti-Estradiol IgG. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Washing Solution 1 x 50ml 17 beta Estradiol Control 1 x 500µl 17 beta Estradiol Standard 0 (0 pg/mL) 1 x 500µl 17 beta Estradiol Standard 1 (20 pg/mL) 1 x 500µl 17 beta Estradiol Standard 2 (120 pg/mL) 1 x 500µl 17 beta Estradiol Standard 3 (300 pg/mL) 1 x 500µl 17 beta Estradiol Standard 4 (600 pg/mL) 1 x 500µl 17 beta Estradiol Standard 5 (2,000 pg/mL) 1 x 500µl 17 beta Estradiol-HRP Conjugate 1 x 22ml Anti-17 beta Estradiol IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108667于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
Mouse and rat samples may need additional optimization. |
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. Mouse and rat samples may need additional optimization. |
图片
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Biological fluids for ab108667
17 beta Estradiol measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-2 fold.
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Typical Standard CurveRepresentative Standard Curve using ab108667
数据表及文件
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SDS download
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Datasheet download
文献 (57)
ab108667 被引用在 57 文献中.
- MacArthur TA et al. Estradiol and Dihydrotestosterone Levels in COVID-19 Patients. Mayo Clin Proc 98:559-568 (2023). PubMed: 36872195
- Lee J et al. Estrogen Mediates the Sexual Dimorphism of GT1b-Induced Central Pain Sensitization. Cells 12:N/A (2023). PubMed: 36899944
- Heger J et al. Sex Difference in Cardioprotection against Acute Myocardial Infarction in MAO-B Knockout Mice In Vivo. Int J Mol Sci 24:N/A (2023). PubMed: 37047416
- Cruz-Cano NB et al. Sex steroid receptors in the ovarian follicles of the lizard Sceloporus torquatus. Zygote 31:386-392 (2023). PubMed: 37212054
- Haque N et al. Deficiency of Adipose Aryl Hydrocarbon Receptor Protects against Diet-Induced Metabolic Dysfunction through Sexually Dimorphic Mechanisms. Cells 12:N/A (2023). PubMed: 37443781