15(S)-HETE ELISA试剂盒(ab133035)
Key features and details
- Sensitivity: 69.21 pg/ml
- Range: 78.1 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
概述
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产品名称
15(S)-HETE ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Low conc 16 181pg/ml = 15.8% Medium conc 16 853pg/ml = 9.8% High conc 16 3929pg/ml = 3.4% 批次间 样品 n Mean SD CV% Low conc 8 192pg/ml = 19.1% Medium conc 8 730pg/ml = 8.9% High conc 8 4335pg/ml = 6.1% -
样品类型
Cell culture supernatant, Urine, Serum, Plasma -
检测类型
Competitive -
灵敏度
= 69.21 pg/ml -
范围
78.1 pg/ml - 20000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Urine = 89.6 % - % Serum = 95.9 % - % Tissue Culture Media = 100.3 % - % EDTA Plasma = 105.4 % - % -
实验步骤
Multiple steps standard assay -
产品概述
Abcam’s 15(S)-HETE in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 15-hydroxyeicosatetraenoic acid in plasma, serum, urine, tissue culture media and other biological fluids.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-15(S)-HETE antigen and a polyclonal rabbit antibody specific to 15(S)-HETE. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 15(S)-HETE captured in the plate.
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说明
15(S)-HETE (15-hydroxyeicosatetraenoic acid) is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase (15-LOX). It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1. Aspirin-mediated acetylation of the COX-1 enzyme results in 15(R)-HETE. Blood platelets, peripheral leukocytes, vascular smooth muscle and other cell types produce Type-1 15-LOX while prostate, lung, skin and cornea tissues produce Type-2. 15-HETE has been proposed to act as a paracrine regulator of smooth muscle and lung neutrophil recruitment due in part to its incorporation into tracheal epithelium at the sn-2 position of phosphatidylinositol. The phosphoinositol modification in turn is thought to affect signal transduction and the regulation of intracellular calcium. Interleukin-4 has been shown to regulate 15(S)-HETE expression and incorporation into cellular phospholipids. 15(S)-HETE binds to actin and the alpha-subunit of mitochondrial ATP synthase suggesting a more direct method in regulating some physiological activities. Increased levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.
Cross Reactivity
Compound % Cross Reactivity 15(S)-HETE 100 5,15-diHETE 1 8,15-diHETE
1 13(S)-HODE 0.6 5-HETE 0.1 PGB2 0.1 PGD2 0.1 PGF2α 0.1 12(S)-HETE <0.05 12(R)-HETE <0.05 Arachidonic Acid <0.05 PGE2 <0.05 Linoleic Acid <0.05 -
经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Please refer to protocols. -
组件 1 x 96 tests 15(S)-HETE Alkaline Phosphatase Conjugate 1 x 5ml 15(S)-HETE Antibody 1 x 5ml 15(S)-HETE Standard 1 x 500µl 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 2 units pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
研究领域
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别名
- 15(S)-hydroperoxy tetraenoic eicosatetraenoic acid
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133035于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Competitive ELISA |
Use at an assay dependent concentration.
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说明 |
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Competitive ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab133035 被引用在 9 文献中.
- Miao Z et al. Hsp90 induces Acsl4-dependent glioma ferroptosis via dephosphorylating Ser637 at Drp1. Cell Death Dis 13:548 (2022). PubMed: 35697672
- Bao Z et al. MEF2C silencing downregulates NF2 and E-cadherin and enhances Erastin-induced ferroptosis in meningioma. Neuro Oncol 23:2014-2027 (2021). PubMed: 33984142
- Bao Z et al. Prokineticin-2 prevents neuronal cell deaths in a model of traumatic brain injury. Nat Commun 12:4220 (2021). PubMed: 34244497
- Du Y et al. Ferroptosis is involved in the anti-tumor effect of lycorine in renal cell carcinoma cells. Oncol Lett 22:781 (2021). PubMed: 34594422
- Cheng J et al. ACSL4 suppresses glioma cells proliferation via activating ferroptosis. Oncol Rep 43:147-158 (2020). PubMed: 31789401
- Tan S et al. Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis. Oncol Lett 20:122 (2020). PubMed: 32863935
- Li Y et al. Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusion. Cell Death Differ N/A:N/A (2019). PubMed: 30737476
- Sandstedt M et al. Hypoxic cardiac fibroblasts from failing human hearts decrease cardiomyocyte beating frequency in an ALOX15 dependent manner. PLoS One 13:e0202693 (2018). PubMed: 30138423
- Vijil C et al. Arachidonate 15-lipoxygenase enzyme products increase platelet aggregation and thrombin generation. PLoS One 9:e88546 (2014). PubMed: 24533104