AB133035

15(S)-HETE ELISA试剂盒

Name: 15(S)-HETE ELISA试剂盒 (ab133035)| Abcam中文官网
Brand: Abcam Limited
SKU: AB133035
Price: 7527 CNY
Availability: InStock

15(S)-HETE ELISA Kit

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(13 Publications)

15(S)-HETE ELISA Kit is a competitive ELISA designed to quantify 15(S)-HETE with a sensitivity of 69.21 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 78.1 - 20000 pg/ml
- Cited in over 10 publications

查看别名

15(S)-hydroperoxy tetraenoic eicosatetraenoic acid

1 Images

Competitive ELISA - 15(S)-HETE ELISA Kit (AB133035)

cELISA

Supplier Data

Competitive ELISA - 15(S)-HETE ELISA Kit (AB133035)

Representative Standard Curve using ab133035

关键信息

检测方法

Colorimetric

样品类型

Urine, Plasma, Cell culture supernatant, Serum

检测类型

Competitive

灵敏度

= 69.21 pg/mL

范围

78.1 - 20000 pg/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Abcam's 15(S)-HETE in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 15-hydroxyeicosatetraenoic acid in plasma, serum, urine, tissue culture media and other biological fluids.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-15(S)-HETE antigen and a polyclonal rabbit antibody specific to 15(S)-HETE. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 15(S)-HETE captured in the plate.

15(S)-HETE (15-hydroxyeicosatetraenoic acid) is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase (15-LOX). It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1. Aspirin-mediated acetylation of the COX-1 enzyme results in 15(R)-HETE. Blood platelets, peripheral leukocytes, vascular smooth muscle and other cell types produce Type-1 15-LOX while prostate, lung, skin and cornea tissues produce Type-2. 15-HETE has been proposed to act as a paracrine regulator of smooth muscle and lung neutrophil recruitment due in part to its incorporation into tracheal epithelium at the sn-2 position of phosphatidylinositol. The phosphoinositol modification in turn is thought to affect signal transduction and the regulation of intracellular calcium. Interleukin-4 has been shown to regulate 15(S)-HETE expression and incorporation into cellular phospholipids. 15(S)-HETE binds to actin and the alpha-subunit of mitochondrial ATP synthase suggesting a more direct method in regulating some physiological activities. Increased levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.

Cross Reactivity

Compound	% Cross Reactivity
15(S)-HETE	100
5,15-diHETE	1
8,15-diHETE	1
13(S)-HODE	0.6
5-HETE	0.1
PGB₂	0.1
PGD₂	0.1
PGF_2α	0.1
12(S)-HETE	<0.05
12(R)-HETE	<0.05
Arachidonic Acid	<0.05
PGE₂	<0.05
Linoleic Acid	<0.05

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精确度

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 8, "mean": "4335 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Intra", "sample": "Medium conc", "replicates": 16, "mean": "853 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 9.8" }, { "reproducibilityType": "Inter", "sample": "Low conc", "replicates": 8, "mean": "192 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 19.1" }, { "reproducibilityType": "Intra", "sample": "Low conc", "replicates": 16, "mean": "181 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 15.8" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 16, "mean": "3929 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 3.4" }, { "reproducibilityType": "Inter", "sample": "Medium conc", "replicates": 8, "mean": "730 pg/mL", "standardDeviation": null, "coefficientOfVariability": "= 8.9" } ]

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回收率

[ { "sample": "EDTA Plasma", "range": null, "average": "= 105.4" }, { "sample": "Serum", "range": null, "average": "= 95.9" }, { "sample": "Tissue Culture Media", "range": null, "average": "= 100.3" }, { "sample": "Urine", "range": null, "average": "= 89.6" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133035-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Plate Sealer", "number":"AB133035-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Goat anti-rabbit IgG Microplate (12 x 8 wells)", "number":"AB133035-CMP09", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133035-CMP08", "productcode":"" }, { "size":"1 x 27 mL", "name":"Assay Buffer", "number":"AB133035-CMP01", "productcode":"" }, { "size":"1 x 500 µL", "name":"15(S)-HETE Standard", "number":"AB133035-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133035-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"15(S)-HETE Alkaline Phosphatase Conjugate", "number":"AB133035-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"15(S)-HETE Antibody", "number":"AB133035-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133035-CMP04", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

Please refer to protocols

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

15(S)-HETE also known as 15(S)-hydroxyeicosatetraenoic acid is a bioactive lipid molecule with a molecular mass of approximately 320.5 g/mol. This lipid derives from arachidonic acid through the enzymatic action of lipoxygenases primarily 15-lipoxygenase (15-LOX). It is expressed in various tissues including the lung skin and inflammatory cells. Its production occurs predominantly in response to cellular conditions that activate lipoxygenase pathways contributing to its role in various bodily processes.

Biological function summary

15(S)-HETE functions as a signaling molecule involved in inflammation and cell growth regulation. It participates in modulating cellular responses including acting on endothelial cells where it influences vascular tone and permeability. 15(S)-HETE is not part of a larger protein complex but it interacts with receptors on the cell surface and intracellular signaling pathways. Its role in inflammatory responses and vascular homeostasis is critical for normal physiological processes.

Pathways

15(S)-HETE contributes significantly to the inflammation and arachidonic acid metabolism pathways. It is closely related to prostaglandin and leukotriene synthesis positioning it as an important player in the regulation of inflammation. Within these pathways it interacts with proteins such as cyclooxygenase enzymes and other lipoxygenase isozymes influencing their activities and the production of other eicosanoids that mediate inflammatory responses.

15(S)-HETE shows connections to asthma and atherosclerosis. In asthma it participates in the regulation of airway inflammation and bronchial reactivity often involving interactions with proteins such as cytokines and eosinophils that exacerbate the inflammatory response. For atherosclerosis it is implicated in plaque formation through its effects on endothelial cells and macrophages highlighting its role in modulating the chronic inflammatory environment associated with the disease.

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产品实验方案

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靶点信息

See full target information 15(S)-HETE

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 15:e70347 PubMed40462502

2025

The deubiquitinase OTUD3 plays a neuroprotective role by reducing ferroptosis induced by cerebral ischaemia reperfusion via stabilizing PLK1 via deubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Cheng,Qi Tian,Hao-Ran Lu,Hong-Xiang Jiang,Xiao-Hong Qin,Yan-Qin Fan,Zhi-Biao Chen,Li-Quan Wu

Molecular biotechnology 67:689-704 PubMed38456959

2024

O-sialoglycoprotein Endopeptidase (OSGEP) Suppresses Hepatic Ischemia-Reperfusion Injury-Induced Ferroptosis Through Modulating the MEK/ERK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Tao,Wanqing Zhou,Cheng Chen,Qian Zhang,Zhuoyi Liu,Pingping Xia,Zhi Ye,Chunling Li

British journal of cancer 130:526-541 PubMed38182686

2024

TRIM21/USP15 balances ACSL4 stability and the imatinib resistance of gastrointestinal stromal tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Cui,Haoyu Sun,Zhishuang Gao,Chao Li,Tingting Xiao,Yibo Bian,Zonghang Liu,Tianhao Gu,Jianan Zhang,Tengyun Li,Qianzheng Zhou,Zhongyuan He,Bowen Li,Fengyuan Li,Zekuan Xu,Hao Xu

Journal of cellular and molecular medicine 28:e17983 PubMed38070189

2023

Inhibition of ferroptosis by POLE2 in gastric cancer cells involves the activation of NRF2/GPX4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Jian,Zhi-Qiang Chen,Heng Du,Ting Liao,Yi-Chen Sun,Dong Ke,Yang Yu

Cell death & disease 13:548 PubMed35697672

2022

Hsp90 induces Acsl4-dependent glioma ferroptosis via dephosphorylating Ser637 at Drp1.

Applications

Unspecified application

Species

Unspecified reactive species

Zong Miao,Wei Tian,Yangfan Ye,Wei Gu,Zhongyuan Bao,Lei Xu,Guangchi Sun,Chong Li,Yiming Tu,Honglu Chao,Sin Man Lam,Ning Liu,Jing Ji

Oncology letters 22:781 PubMed34594422

2021

Ferroptosis is involved in the anti-tumor effect of lycorine in renal cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Du,Hong-Chao Zhao,Heng-Cheng Zhu,Yao Jin,Lei Wang

Nature communications 12:4220 PubMed34244497

2021

Prokineticin-2 prevents neuronal cell deaths in a model of traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyuan Bao,Yinlong Liu,Binglin Chen,Zong Miao,Yiming Tu,Chong Li,Honglu Chao,Yangfan Ye,Xiupeng Xu,Guangchi Sun,Pengzhan Zhao,Ning Liu,Yan Liu,Xiaoming Wang,Sin Man Lam,Valerian E Kagan,Hülya Bayır,Jing Ji

Neuro-oncology 23:2014-2027 PubMed33984142

2021

MEF2C silencing downregulates NF2 and E-cadherin and enhances Erastin-induced ferroptosis in meningioma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyuan Bao,Lingyang Hua,Yangfan Ye,Daijun Wang,Chong Li,Qing Xie,Hiroaki Wakimoto,Ye Gong,Jing Ji

Oncology letters 20:122 PubMed32863935

2020

Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shougang Tan,Xiaoqun Hou,Lin Mei

Oncology reports 43:147-158 PubMed31789401

2019

ACSL4 suppresses glioma cells proliferation via activating ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Cheng,Yan-Qin Fan,Bao-Hui Liu,Han Zhou,Jun-Min Wang,Qian-Xue Chen

View all publications

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15(S)-HETE ELISA试剂盒

15(S)-HETE ELISA Kit

Competitive ELISA - 15(S)-HETE ELISA Kit (AB133035)