11-dehydro-TXB2 ELISA试剂盒(ab133054)
Key features and details
- Sensitivity: 4.31 pg/ml
- Range: 9.8 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
11-dehydro-TXB2 ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Low conc 16 67pg/ml 13.2% Medium conc 16 165pg/ml 15.3% High conc 16 318pg/ml 8.1% 批次间 样品 n Mean SD CV% Low conc 8 72pg/ml 17.5% Medium conc 8 216pg/ml 12.6% High conc 8 1250pg/ml 16.3% -
样品类型
Cell culture supernatant, Urine -
检测类型
Competitive -
灵敏度
4.31 pg/ml -
范围
9.8 pg/ml - 10000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Urine 112.2 % - % Tissue Culture Media 104.8 % - % -
检测时间
3h 0m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human
预测可用于: a wide range of other species -
产品概述
Abcam’s 11-dehydro-TXB2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 11-dehydro-TXB2 in tissue culture media and urine.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-11-dehydro-TXB2 antigen and a polyclonal rabbit antibody specific to 11-dehydro-TXB2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 11-dehydro-TXB2 captured in the plate.
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说明
11-dehydro-Thromboxane B2 (11-dehydro-TXB2) is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma. It is produced by the dehydrogenation of the alcohol group on the C11 position of TXB2 by the enzyme 11-OH-dehydrogenase. The detection of 11-dehydro-TXB2 is often used to measure thromboxane production in vivo. Monitoring levels of this metabolite is a helpful tool in the study of several diseases, such as liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and other diseases involving platelet activation.11-dehydro-TXB2 has also been used in diabetes and asthma studies.
Cross reactivities:
Compound Cross Reactivity 11-dehydro-TXB2 100% PGE2 1.85% TXB2 0.4% PGF2α 0.2% 6-keto-PGF1α 0.17% PGD2 0.1% 2,3-dinor TXB2 0.1% 8-iso-PGF2α 0.07% 11-b-PGF2α 0.05% -
经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
-
存放说明
Please refer to protocols. -
组件 1 x 96 tests 11-dehydro-Thromboxane B2 Standards 1 x 500µl 11-dehydro-TXB2 Alkaline Phosphatase Conjugate 1 x 6ml 11-dehydro-TXB2 Antibody 1 x 6ml 20X Wash Buffer Concentrate 1 x 30ml Assay Buffer 1 x 30ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
研究领域
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别名
- 11-dehydro-Thromboxane B2
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133054于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Competitive ELISA |
Use at an assay dependent concentration.
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说明 |
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Competitive ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133054 被引用在 1 文献中.
- Liu D et al. Chemokine CC-motif ligand 2 participates in platelet function and arterial thrombosis by regulating PKCa-P38MAPK-HSP27 pathway. Biochim Biophys Acta N/A:N/A (2018). ELISA . PubMed: 29864522