Fluorescent SimpleStep ELISA® kit
Fluorescent ELISA kits provide a highly sensitive and quantitative approach to analyte detection, using fluorescence-based readouts for improved accuracy and dynamic range compared to colorimetric methods. CatchPoint SimpleStep ELISA kits have been developed using a fluorescent substrate to provide improved linearity over an extended dynamic range when compared to the TMB substrate, the most widely used colorimetric substrate for ELISA. The extended dynamic range provides better quantification at both the lower and the upper end of the curve, as shown in the examples below.
Benefits of CatchPoint® SimpleStep® ELISA
- One-wash, 90-minute protocol: save precious time with a standardized and simplified protocol thanks to our innovative technology. Learn more about how our technology works here.
- A stable signal that provides readout flexibility: the signal is stable for up to 24 hours, providing you with the flexibility to read the results at your convenience.
- No compromise on sensitivity: assay development is focused on achieving the highest sensitivity possible for each kit.
- Made with high-quality antibodies for batch-to-batch consistency: antibody production is controlled and reliable leading to improved consistency and reproducibility.
How to use CatchPoint SimpleStep ELISA kits
CatchPoint SimpleStep ELISA kits are optimized for Molecular Devices Multi-Mode Microplate Readers.
Take a look at the Molecular Devices application note for more information on how you can achieve improved performance of CatchPoint SimpleStep ELISA kits on Molecular Devices’ SpectraMax®.
CatchPoint SimpleStep ELISA example data
The example below show the improved linearity over an extended dynamic range of our Human ICAM-1 (ab229383) CatchPoint SimpleStep ELISA kit in comparison to equivalent SimpleStep ELISA kits (ab174445) that use the TMB colorimetric substrate.
Figure 1. Standard curve comparison over the extended dynamic range of Human ICAM-1 CatchPoint SimpleStep ELISA kit (ab229383) vs. Human ICAM-1 SimpleStep ELISA kit (ab174445). The fluorescent ELISA kit (ab229383) shows better linearity than the colorimetric ELISA kit (ab174445) over the extended dynamic range.