55分钟化学发光 SimpleStep Ignite™ ELISA 试剂盒
‣ 50× greater sensitivity and up to 35× broader measuring range.
‣ Results in ~55 minutes, that's 6x faster than the industry standard, making SimpleStep Ignite™ the fastest chemiluminescent ELISA on the market.
‣ Consistent, reproducible performance batch after batch.
‣ Simple, standardized protocol with only one incubation and a single wash step.
Save 50% on your first SimpleStep Ignite™ ELISA kit. Enter the code IGNITE50 at checkout. See the range. Discount valid until 31 October 2026. T&Cs apply.
What is a chemiluminescent ELISA?
Chemiluminescent ELISA kits use light-emitting reactions to offer highly sensitive and rapid detection of biomarkers.
These kits use a chemiluminescent substrate to produce light in response to antigen-antibody interactions, which allows for greater sensitivity and broader dynamic range than traditional ELISA. Our 90‑minute colorimetric SimpleStep® ELISA kits have already saved researchers around the world 82 years at the bench. SimpleStep Ignite™ ELISA builds on this legacy - helping accelerate the pace of scientific discovery.
What are the benefits of SimpleStep Ignite ELISA?
什么是SimpleStep IgniteTM ELISA试剂盒？
SimpleStep IgniteTM ELISA试剂盒采用化学发光反应和一步法检测结合来提高灵敏度和快速生物标志物检测。
SimpleStep IgniteTM ELISA采用经过优化的 重组抗体对 制成，并已在多种生物样本中完成验证，包括血清、血浆、细胞培养上清液和脑脊液，确保试剂盒批次之间始终如一的稳定性。
90 分钟比色法SimpleStep ELISA®试剂盒已将检测效率提升至传统 ELISA 的约 3 倍。为进一步突破检测速度，Abcam推出了化学发光SimpleStep IgniteTM ELISA试剂盒，将总孵育时间缩短至仅30分钟，整体检测时间缩短至55分钟，实验速度较传统ELISA提升约6倍。
SimpleStep IgniteTM 是如何工作的？
沿用 SimpleStep®特有的“一步法”流程：混合→单洗→读数，大幅降低操作误差，提高实验可重复性。
- 加入样本和抗体预混液：将样本与所有检测抗体（包括捕获抗体和抗标签抗体）一次性混合孵育 30 分钟。
- 洗涤后加入化学发光底物：洗去未结合物质，加入底物反应 2 分钟。
- 读板：立即在化学发光读板机上读取信号。
图 1：SimpleStep Ignite™ ELISA试剂盒单次洗涤的标准化工作流程
SimpleStep IgniteTM ELISA试剂盒的优势
Watch the protocol
Discover how easy SimpleStep Ignite™ ELISA is in practice, from setup to signal, with a streamlined, single-wash protocol and minimal handling.
‣ Prepare samples, standards, and reagents
‣ Add the antibody cocktail and incubate
‣ Wash once
‣ Add the development solution and read
Watch the video to see the step-by-step protocol in action.
Making it easier to switch ELISA platforms
Switching assays mid-project can feel like a risk. That's why SimpleStep Ignite™ ELISA was designed to make validation, adoption, and scaling as straightforward as possible. The combination of enhanced sensitivity, broad measuring range, and a familiar one-wash workflow helps you transition with confidence while minimizing disruption to ongoing work.
Validate with confidence
Evaluating a new assay shouldn't require a large upfront commitment.
If you're new to SimpleStep Ignite™ ELISA, you can get 50% off your first kit, making it easier to assess performance in your own samples and workflows before wider adoption.
Scale as your project grows
Whether you're running early-stage experiments or supporting larger biomarker programs, SimpleStep Ignite™ ELISA is designed to support your long-term needs.
- Manufactured in-house for greater control over quality and supply
- Multiple pack sizes available, including 10-pack options
- Future expansion plans, including 384-well formats
- Support for ongoing project and volume requirements
Reliable supply for ongoing studies
Consistency matters when projects move from validation to routine testing.
Our team can work with you to discuss stocking programs and supply planning, helping ensure you have the assays you need as your research scales.
Support every step of the way
From initial evaluation through implementation, our scientific and commercial teams can help you:
- Compare assay options
- Evaluate suitability for your application
- Plan validation studies
- Discuss scaling and supply requirements
- Identify the best solution for your project goals
See the benefits for yourself
Let’s discuss your ELISA project
Frequently asked questions
How do chemiluminescent ELISA kits differ from traditional colorimetric ELISA kits?
Chemiluminescent ELISA kits use a luminescent substrate that emits light as a signal, which is then measured via a luminometer. This method generally provides higher sensitivity and a broader dynamic range compared to the colorimetric method, which relies on a chromogenic substrate and optical density measurements.
Are these kits suitable for all types of samples?
Chemiluminescent ELISA kits can be used with a variety of sample types, including serum, plasma, cell culture supernatants, and other biological fluids. However, it's important to consider a specific kit's compatibility with your sample type for optimal results.
What equipment is needed to use a chemiluminescent ELISA kit?
You will need a plate reader equipped to detect chemiluminescence.
See the performance difference
Traditional ELISA methods often require compromises between sensitivity, assay time and measuring range.
SimpleStep Ignite™ ELISA kits are designed to help overcome these challenges. The examples below demonstrate how chemiluminescent detection improves assay performance, delivering enhanced signal-to-noise ratios and greater sensitivity across multiple targets compared with conventional colourimetric ELISA.
Higher sensitivity
Detect low-abundance analytes with confidence.
Across multiple assays, chemiluminescent readout improves the signal-to-noise ratio, increasing analytical sensitivity and precision (Figure 1).
The data in the graphs show enhanced signal-to-noise performance and stronger detection at lower analyte concentrations, helping researchers achieve reliable quantification of low-abundance targets.
Chemiluminescent detection can achieve substantially greater sensitivity than conventional colourimetric approaches, making it particularly valuable when sample availability is limited or target concentrations are low (Figures 2a and 2b).
Figure 1: Signal-to-noise improvement with SimpleStep Ignite™
Comparison of the signal-to-noise ratios for Human IL‑4, measured by chemiluminescent (orange) and colorimetric (blue) detection. Chemiluminescent readout improves low‑end signal-to-noise, thereby increasing analytical sensitivity and precision
Figure 2a: Enhanced sensitivity for Human Pro-Collagen I alpha 1 detection
Figure 2b: Enhanced sensitivity for Human TNF alpha detection
Broader measuring range
More measurable results. Fewer reruns.
A broader measuring range allows accurate quantification across a wider concentration spectrum, reducing the need for repeated testing and sample dilution.
We compared reported Human C-peptide concentrations from 450 samples and found that all fell within the measuring range of SimpleStep Ignite™, demonstrating its ability to support first-run success across a broad range of sample concentrations.
This helps increase the likelihood that more samples can be measured first time, supporting a smoother workflow with fewer repeats and less hands-on time.
- Wider dynamic range reduces out-of-range results
- Fewer re-tests lead to faster data delivery
- Improved success rate for unknown sample concentrations
Figure 3: Broader measuring range for human C-peptide
Faster results
Improved assay performance should not require longer workflows.
While many commercially available ELISA assays require several hours to complete, SimpleStep Ignite™ ELISA delivers results in just 55 minutes using a single-incubation, single-wash protocol.
- Faster experimental turnaround
- Reduced hands-on time
- More assays completed per day
- Accelerated decision-making
- Improved laboratory productivity
Figure 4: Faster results with SimpleStep Ignite™ ELISA
Comparison of assay workflow times. SimpleStep Ignite™ ELISA delivers results in 55 minutes using a single-incubation, single-wash protocol, enabling faster turnaround than selected alternative ELISA workflows.
SimpleStep IgniteTM ELISA试剂盒与比色法SimpleStep® ELISA试剂盒对比
图2：SimpleStep Ignite™ ELISA试剂盒（橙色条）（ab325360）与比色法SimpleStep® ELISA试剂盒以及其他比色法试剂盒的对比。（SSE： SimpleStep® ELISA）
在检测 Human Pro-Collagen I alpha 1 靶标时，Abcam SimpleStep IgniteTM ELISA 试剂盒展现出优于传统比色法 SimpleStep ELISA（SSE）试剂盒及其他竞品的性能表现，具有更宽的动态检测范围和更高的灵敏度，可实现更低水平靶标的精准检测。
FAQ
1，化学发光 SimpleStep IgniteTM ELISA 试剂盒与传统比色法 ELISA 试剂盒有何不同？
化学发光 SimpleStep IgniteTM ELISA 试剂盒采用化学发光底物产生光信号，并通过化学发光检测仪进行读取而传统比色法ELISA试剂盒在酶的催化作用下将显色底物转化为有色产物，并通过颜色强度对目标物进行定量分析。
先进的化学发光底物能够显著提高信号灵敏度、降低背景噪音，从而更有效地检测低丰度生物标志物，实现更宽的动态检测范围和更低的检测下限。
2，化学发光 SimpleStep IgniteTM ELISA 试剂盒是否适用于所有类型的样品？
化学发光 SimpleStep IgniteTM ELISA 试剂盒可与各种样本类型一起使用，包括血清、 血浆、 细胞培养上清液和其他常见生物体液。在药物研发、转化医学及疾病标志物研究中灵活应用。
请根据具体试剂盒确认样本类型的兼容性，以获得最佳实验结果。
3，使用化学发光 SimpleStep IgniteTM ELISA 试剂盒需要什么仪器？
您需要一台支持化学发光读数的板式读数仪（luminometer）。