SimpleStep ELISA® kits

SimpleStep ELISA kits are streamlined immunoassays designed to simplify and accelerate the traditional ELISA workflow. By combining capture and detection antibodies into a single reagent and reducing the protocol to a single wash step, these kits offer faster results with fewer handling errors, making them ideal for researchers seeking efficiency without compromising sensitivity. Each kit incorporates recombinant monoclonal antibodies for high batch-to-batch consistency and is rigorously validated on relevant biological samples to ensure reliable performance in real-world applications.

SimpleStep ELISAs are available in both sandwich and competitive formats, and are suitable for a wide range of targets, from large proteins to small molecules, such as hormones and epitope tags. For labs requiring higher throughput, many kits are also offered in a 384-well plate format, enabling rapid screening of large sample sets with minimal reagent use.

Figure 1. With SimpleStep ELISA kits, an analyte-capture and detector antibody sandwich complex is formed in solution, which binds to the microplate via an affinity tag attached to the capture antibody in the sandwich pair.

Play video to see the SimpleStep ELISA® protocol in action.

Colorimetric SimpleStep ELISA

Available as sandwich or competitive ELISA kits, our colorimetric 90-minute SimpleStep kits are compatible with absorbance plate readers.

Fluorescent SimpleStep ELISA

CatchPoint SimpleStep ELISA kits have been developed using a fluorescent substrate to provide improved linearity over an extended dynamic range