AB102918

PE / R-Phycoerythrin偶联试剂盒 - Lightning-Link®

Name: PE / R-Phycoerythrin Conjugation试剂盒- Lightning-Link® (ab102918)| Abcam中文官网
Brand: Abcam Limited
SKU: AB102918
Price: 3101 CNY
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Rating: 5 (2 reviews)

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®

(2 Reviews)

(136 Publications)

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) offers several standout features:

- Rapid Conjugation: achieve PE / R labeling in under 4 hours with just 30 seconds of hands-on time.
- High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Versatility: suitable for conjugating antibodies, proteins, and peptides. PE / R labeled antibodies can be used immediately in applications such as Flow cytometry, ELISA, and Immunohistochemistry (IHC) without further purification.
- Confidence: cited in over 115 publications.

13 Images

Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Flow Cyt

PubMed

Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit Lightning-Link.

Robinson, Andrew P., et al used PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) as part of characterizing oligodendroglial populations. They used the kit to conjugate PE / R-Phycoerythrin to Mouse monoclonal anti-GALC antibody for use in flow cytometry.
SJL/J mice were immunized with PLP_139-151 and scored daily for clinical disease. A cohort of SJL/J mice was sacrificed, and spinal cords were analyzed by flow cytometry (n=5). (A) Cells were distinguished from debris by forward and side scatter then singlet cells were gated. Live cells were gated by dead cell exclusion, and CNS resident cells were identified as CD45^- or CD45^low. (B) Oligodendroglial cells were defined by double positive staining : A2B5+PDGFRalpha⁺ early OPCs, A2B5+NG2+ intermediate OPCs, NG2+O4+ late OPCs, O4+MOG+ pre-myelinating oligodendrocytes, and GALC+MOG+ mature oligodendrocytes.

Image from Robinson, Andrew P., et al., PloS one, 9(9):e107649. doi: 10.1371/journal.pone.0107649. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Schematic Diagram - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Schematic Diagram

Supplier Data

Schematic Diagram - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

This illustration demonstrates a general procedure of how Lightning-Link® labeling technology enables the direct labeling of antibodies or proteins.

Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for around 3 hours. Please see the ab102918 protocol booklet for more details.

Learn more about our Lightning-Link® conjugation kits here

Flow Cyt

AbReview38946****

Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Flow cytometry using antibody conjugated using EasyLink R-Phycoerythrin conjugation kit (ab102918).

Flow cytometry histogram showing integrin beta-3 positive population of platelets from wildtype and knockout mice. Integrin beta-3 antibody was conjugated using EasyLink R-Phycoerythrin conjugation kit (ab102918). Flow cytometry was performed using platelets from wild type and integrin beta-3 knockout mice. Mice that expressed beta-3 (shown in red) had a clear shift in FL-2 fluorescence over beta-3 knockout mice (shown in black).

This image is courtesy of an anonymous Abreview.

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Conjugation

PubMed

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Ronnberg E et al. conjugated PE to an anti-chymase antibody with ab102918 PE Conjugation Kit as part of investigating the role of IL-33 in mast cells.
The mean fluorescence intensity of chymase expression in cells was measured by intra-cellular flow cytometry staining and normalized to the respective isotype control.

This data shows that there was no significant change in the expression on chymase in cord blood-derived mast cells (CBMC) when treated with different combinations of IL-33 and TSLP.

Image from Rönnberg E et al., Frontiers in immunology., 10, 1361.. Fig 4C.; doi: 10.3389/fimmu.2019.01361 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/.

Coagulation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Coag

PubMed

Coagulation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling anti-MUC1 SP antibodies FACS.

Kovjazin R et al. used ab102918 to evaluate the cell surface presence of the MUC1 SP domain by FACS.

The cell surface presence of the MUC1 SP domain was evaluated by gated FACS analysis on PC cells in fresh BM aspirates obtained from MM patients (A–E) and normal naive sample (F–H).

Image from Kovjazin R et al., PLoS One, 9(1):e85400. Fig 3.; doi: 10.1371/journal.pone.0085400. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Conjugation

PubMed

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling SPmAb-6 antibody for Imagestream analysis.

Kovjazin R et al. used ab102918 for detection of MUC1 SP on the membrane of MUC1-positive tumor cells.

image from Kovjazin R et al., PLoS One, 9(1):e85400. Fig 2.; doi: 10.1371/journal.pone.0085400. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Conjugation

PubMed

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling A20 antibody for FACS.

Hjelmeland AB et al. used ab102918 as part of an experiment studying the mechanisms that cause A20 regulator to have differential effects on tumor growth and cancer cell behaviors.

They used the PE labelling kit to conjugate phycoerythrin to A20 antibody for use in FACS.

FACS plots are shown for T08-837 (A) and CW468 (B).

Image from Hjelmeland AB et al., PLoS Biol., 8(2):e1000319. Fig 2.; doi: 10.1371/journal.pbio.1000319.Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Conjugation

PubMed

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Tan H-X et al. labeled recombinant influenza A H1N1 NP protein with PE or APC fluorochromes using ab102918 PE / R-Phycoerythrin Conjugation Kit and ab201807 APC Conjugation Kit.

Representative HA and NP probe staining in flow cytometry of GC B cells (B220+ IgD- CD38lo GL7+) isolated from lung inducible bronchus-associated tissues (iBALT), mediastinal LN (MLN), and spleen of mice at d35 post-infection with A/Puerto Rico/8/34 (PR8).

Image from Tan HX et al., Frontiers in immunology., 10, 611.. Fig 4A.; doi: 10.3389/fimmu.2019.00611 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Conjugation

PubMed

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling F(ab')2 antibody for Bead array.

Ayoglu B et al. used ab102918 to detect complement activation driven C3 deposition on beads.

For measuring the background, classical, lectin & alternative and only alternative pathway activation, a serially diluted serum sample (1∶1–1∶160) was applied to empty, human IgG. The assay buffer for serum dilution contained either Ca2+-Mg2+, which promotes complement activation, or EDTA, which blocks complement activation. Plot displays for each serum dilution the respective median fluorescence intensity (MFI) value against varying concentrations of human IgG.

Image from Ayoglu B et al., PLoS One 9(5):e96403. Fig 2.; doi: 10.1371/journal.pone.0096403. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Conjugation

PubMed

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

PE conjugation kit used to label DDR2 antibody for Flow Cytometry.

RA Bagchi et al used PE conjugation kit / PE labeling kit ab102918 as part of examining the conversion of fibroblasts to myofibroblasts. They used the PE labeling kit to conjugate phycoerythrin to a DDR2 antibody for use in flow cytometry.

Charts are forward-scatter plots illustrating flow cytometry analysis of cardiac cells from WT and scleraxis KO mice. Left column, unstained cells; center column, stained cells from WT tissue; right column, stained cells from scleraxis KO tissue. Results are representative of assessments from n = 3 independent tissue samples. Purple outline denotes labeled cells, and is derived from unstained plots.

Bagchi RA et al. BMC Biol. 2016 Mar 17;14:21 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Conjugation

PubMed

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling anti-mouse insulin antibody for Flow Cytometry.

Kalis M et al. used ab102918 as part of examining beta-cells deletion of Dicer1.

They used the kit to conjugate PE to anti-mouse insulin antibody for use in flow cytometry.

Islet cells suspension from 50 islets from 8 weeks old littermates and RIP-Cre Dicer1Δ/Δ mice were stained for insulin and glucagon and analyzed by flow cytometry. Side scatter (SSC) and forward scatter (FSC) analysis of islet cells and the respective gate used to identify live cells. Littermate control islet cells contained significantly more insulin-positive cells (P<0.001) and less glucagon-positive cells (P<0.05) than the RIP-Cre Dicer1Δ/Δ islet cells. Representative of 3 independent experiments.

Image from Kalis, M et al., PLoS One, 6(12):e29166. Fig 5.; doi: 10.1371/journal.pone.0029166. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Conjugation

PubMed

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling a panel of antibodies for Microsphere immunoassay based on Luminex.

Charlermroj R et al. used ab102918 as part of an experiment studying four different plant pathogens : a fruit blotch bacterium Acidovorax avenae subsp. citrulli (Aac), chilli vein-banding mottle virus (CVbMV, potyvirus), watermelon silver mottle virus (WSMoV, tospovirus serogroup IV) and melon yellow spot virus (MYSV, tospovirus).

(A-G) X-axis is antibody-coated microsphere and y-axis is median fluorescent intensity (MFI) from each RPE-labeled antibody. (H) Summary of selected antibody pair sets for the detection of the four plant pathogens.

Image from Charlermroj R et al., PloS One, 8(4):e62344. Fig 2.; doi: 10.1371/journal.pone.0062344. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Conjugation

PubMed

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling ADRB2 antibody for Flow Cytometry.

Bigler MB et al. used ab102918 to assess expression of the epinephrine receptor (ADRB2) on CD3- CD56+ lymphocytes by flow cytometry.

Image from Bigler MB et al., PloS One, 10(12):e0145635. Fig 3.; doi: 10.1371/journal.pone.0145635. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

关键信息

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产品详情

R-PE Conjugation Kit/ R-PE Labeling Kit ab102918 uses a simple and quick process for PE labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. To conjugate an antibody to R-PE using this kit:
- add modifier to antibody and incubate for 3 hrs
- add quencher and incubate for 30 mins

The PE conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use. Learn about buffer compatibility below. Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link^® R-PE Labeling Kit. 703-0004 is the same as the 3 mg size. 703-0003 is the same as the 5 x 600 ug size. 703-0010 is the same as the 3 x 60 ug size. 703-0030 is the same as the 3 x 10 ug size. 703-0015 is the same as the 600 μg size. 703-0005 is the same as the 60 μg size.

Amount and volume of antibody for conjugation to R-PE

Kit size	Recommended amount of antibody	Maximum antibody volume¹
3 x 10 μg	3 x 10 μg	3 x 10 μL
60 μg	1 x 60 μg	1 x 60 μL
3 x 60 μg	3 x 60 μg	3 x 60 μL
600 μg	1 x 600 μg	1 x 600 μL
5 x 600 μg	5 x 600 μg	5 x 600 μL
3 mg	1 x 3 mg	1 x 3 mL

The selling size of this product is now based on the amount of antibody that can be conjugated with the kit; the amount of antibody advised that can be used with the kit has also been updated to reflect what will give the best conjugation results. The quantity and formulation of reagents provided have not changed, if you have been previously using the kit successfully with a different amount of antibody, there is no need to change the way that you are using the kit.

¹Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

Buffer Requirements for Conjugation

Buffer should be pH 6.5-8.5.

Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

50mM / 0.6% Tris¹	0.1% BSA	50% glycerol
0.1% sodium azide	PBS	Potassium phosphate
Sodium chloride	HEPES	Sucrose
Sodium citrate	EDTA	Trehalose

¹ Tris buffered saline is almost always ≤ 50 mM / 0.6%

Incompatible buffer constituents

Thiomerosal	Proclin	Glycine
Arginine	Glutathione	DTT

If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.

Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

Storing and handling conjugation kits

Lyophilized Lightning-Link^® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

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规格

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性能和储存信息

运输条件

Ambient - Cannot Ship with Ice

储存信息

-20°C

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产品实验方案

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靶点信息

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文献 (136)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:244 PubMed40542350

2025

Effect of anti-CD4 mAb induced by inhibiting B cell disorder on immune reconstruction of HIV-infected immunological non-responders.

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Unspecified reactive species

Yi Ouyang,Kang Wu,Lei Fu,Panpan Yi,Da Cheng,Xiaoyu Fu

Nature 644:790-798 PubMed40533564

2025

Kupffer cell programming by maternal obesity triggers fatty liver disease.

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Unspecified reactive species

Hao Huang,Nora R Balzer,Lea Seep,Iva Splichalova,Nelli Blank-Stein,Maria Francesca Viola,Eliana Franco Taveras,Kerim Acil,Diana Fink,Franzisca Petrovic,Nikola Makdissi,Seyhmus Bayar,Katharina Mauel,Carolin Radwaniak,Jelena Zurkovic,Amir H Kayvanjoo,Klaus Wunderling,Malin Jessen,Mohamed H Yaghmour,Lukas Kenner,Thomas Ulas,Stephan Grein,Joachim L Schultze,Charlotte L Scott,Martin Guilliams,Zhaoyuan Liu,Florent Ginhoux,Marc D Beyer,Christoph Thiele,Felix Meissner,Jan Hasenauer,Dagmar Wachten,Elvira Mass

Nature communications 16:1200 PubMed39885150

2025

Rhesus Cytomegalovirus-encoded Fcγ-binding glycoproteins facilitate viral evasion from IgG-mediated humoral immunity.

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Claire E Otero,Sophia Petkova,Martin Ebermann,Husam Taher,Nessy John,Katja Hoffmann,Angel Davalos,Matilda J Moström,Roxanne M Gilbride,Courtney R Papen,Aaron Barber-Axthelm,Elizabeth A Scheef,Richard Barfield,Lesli M Sprehe,Savannah Kendall,Tabitha D Manuel,Teresa Beechwood,Linh Khanh Nguyen,Nathan H Vande Burgt,Cliburn Chan,Michael Denton,Zachary J Streblow,Daniel N Streblow,Alice F Tarantal,Scott G Hansen,Amitinder Kaur,Sallie Permar,Klaus Früh,Hartmut Hengel,Daniel Malouli,Philipp Kolb

Cell reports 43:114938 PubMed39488830

2024

An IL-2 mutein increases regulatory T cell suppression of dendritic cells via IL-10 and CTLA-4 to promote T cell anergy.

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Braxton L Jamison,Matthew Lawrance,Chun Jing Wang,Hannah A DeBerg,Lauren J Ziegler,David M Sansom,Marc A Gavin,Lucy S K Walker,Daniel J Campbell

Immuno 4:108-124 PubMed39391865

2024

A Serological Multiplexed Immunoassay (MIA) Detects Antibody Reactivity to SARS-CoV-2 and Other Viral Pathogens in Liberia and Is Configurable as a Multiplexed Inhibition Test (MINT).

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Brien K Haun,Albert To,Caitlin A Williams,Aquena Ball,Karalyn Fong,Teri Ann S Wong,Bode Shobayo,Julius Teahton,Lauren Ching,Varney Kamara,Davidetta M Tekah,Peter Humphrey,John Berestecky,Vivek R Nerurkar,Axel T Lehrer

ImmunoHorizons 8:729-739 PubMed39330967

2024

B Cells Influence Encephalitogenic T Cell Frequency to Myelin Oligodendrocyte Glycoprotein (MOG)38-49 during Full-length MOG Protein-Induced Demyelinating Disease.

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Michael A Faust,Lisa Gibbs,Juan M Oviedo,Douglas H Cornwall,Keke C Fairfax,Zemin Zhou,Tracey J Lamb,Brian D Evavold

iScience 27:110613 PubMed39224516

2024

CelltypeR: A flow cytometry pipeline to characterize single cells from brain organoids.

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Rhalena A Thomas,Julien Sirois,Shuming Li,Alexandre Gestin,Ghislaine Deyab,Valerio E C Piscopo,Paula Lépine,Meghna Mathur,Carol X-Q Chen,Vincent Soubannier,Taylor M Goldsmith,Lama Fawaz,Thomas M Durcan,Edward A Fon

Nature communications 15:7141 PubMed39164224

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CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastoma.

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Guillem Pascual-Pasto,Brendan McIntyre,Margaret G Hines,Anna M Giudice,Laura Garcia-Gerique,Jennifer Hoffmann,Pamela Mishra,Stephanie Matlaga,Simona Lombardi,Rawan Shraim,Patrick M Schürch,Mark Yarmarkovich,Ted J Hofmann,Fatemeh Alikarami,Daniel Martinez,Matthew Tsang,Luis Gil-de-Gómez,Timothy T Spear,Kathrin M Bernt,Adam J Wolpaw,Dimiter S Dimitrov,Wei Li,Kristopher R Bosse

Journal of extracellular vesicles 13:e12474 PubMed39001704

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Characterisation of LPS+ bacterial extracellular vesicles along the gut-hepatic portal vein-liver axis.

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Heetanshi Jain,Ashish Kumar,Sameh Almousa,Shalini Mishra,Kendall L Langsten,Susy Kim,Mitu Sharma,Yixin Su,Sangeeta Singh,Bethany A Kerr,Gagan Deep

The Journal of clinical investigation 134: PubMed38954588

2024

Inhibiting the NADase CD38 improves cytomegalovirus-specific CD8+ T cell functionality and metabolism.

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Nils Mülling,Felix M Behr,Graham A Heieis,Kristina Boss,Suzanne van Duikeren,Floortje J van Haften,Iris N Pardieck,Esmé Ti van der Gracht,Ward Vleeshouwers,Tetje C van der Sluis,J Fréderique de Graaf,Dominique Mb Veerkamp,Kees Lmc Franken,Xin Lei,Lukas van de Sand,Sjoerd H van der Burg,Marij Jp Welters,Sebastiaan Heidt,Wesley Huisman,Simon P Jochems,Martin Giera,Oliver Witzke,Aiko Pj de Vries,Andreas Kribben,Bart Everts,Benjamin Wilde,Ramon Arens

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PE / R-Phycoerythrin偶联试剂盒 - Lightning-Link®

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®

Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Schematic Diagram - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Coagulation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

Conjugation - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

关键信息

产品详情