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小鼠TCS抗体Purification试剂盒(ab128749)

Submit a review Q&A (3)References (5)
Mouse TCS Antibody Purification Kit (ab128749)
  • Mouse TCS Antibody Purification Kit (ab128749)

概述

  • 产品名称

    小鼠TCS抗体Purification试剂盒
    参阅全部 Antibody Purification 试剂盒

  • 产品概述

    Efficient mouse antibody purification from tissue culture supernatants. Processes 10-25 mL of supernatant to purify up to 1.5 mg of antibody per run.


    The mouse resin has a very high affinity and specificity for mouse IgG molecules. ab128749 Mouse TCS Antibody Purification Kit can be used to purify mouse IgG fractions from hybridoma supernatants. The binding strength of bovine IgG to Abcam's Mouse resin is negligible; therefore, it can be used to selectively recover mouse IgG from TCS samples.


    The method involves capture of the mouse antibody on the Mouse resin and removal of unwanted substances using a simple wash procedure. The purified product is eluted and neutralized.


    Antibodies purified using the Mouse TCS Antibody Purification kit are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit, however they are not compatible with our nanoparticle Antibody conjugation kits. To purify antibodies for use with our nanoparticle conjugation kits (Gold, Latex, Europium and Magnetic), please use our Mouse Antibody Purification Kit - Nanoparticles ab204910.


    ab128749 is suitable for use with Mouse antibodies. It is not suitable for use with antibodies from other species.

  • 说明

    This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ Mouse TCS Antibody Purification System. 832-0500 is the same as the 3 tests size. 832-0005 is the same as the 1 test size.

     

性能

  • 存放说明

    Store at +4°C. Please refer to protocols.
  • 组件 1 tests 3 tests
    Binding Buffer (10X) 1 x 6ml 1 x 18ml
    Elution Buffer 1 x 5ml 1 x 15ml
    Mouse Resin 1 x 2ml 3 x 2ml
    Neutralizer Buffer 1 x 2ml 1 x 5ml
    ab274180 - Purification Column 1 unit 3 units
    Spin Cartridge / Collecting Tube Assemblies 1 unit 3 units
    Wash Buffer 1 x 25ml 1 x 75ml

  • 研究领域

图片

  • Mouse TCS Antibody Purification Kit (ab128749)
    Mouse TCS Antibody Purification Kit (ab128749)

    A representative bar graph comparing the effectiveness of Mouse TCS Antibody Purification Kit and Protein G resin.

  • Mouse TCS Antibody Purification Kit (ab128749)
    Mouse TCS Antibody Purification Kit (ab128749)

    Gel electrophoresis of TCS samples purified with the Mouse TCS Antibody Purification Kit.

    M = Mouse IgG1 in a TCS sample

    B = Bovine serum IgG in a TCS sample

    TCS = Tissue culture supernatant sample

文献 (5)

发表研究结果有使用 ab128749？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab128749 被引用在 5 文献中.

  • Wherry TLT  et al. Vitamin D3 alters macrophage phenotype and endosomal trafficking markers in dairy cattle naturally infected with Mycobacterium avium subsp. paratuberculosis. Front Cell Infect Microbiol 12:1021657 (2022). PubMed: 36275033
  • Melandri D  et al. The ?dTCR combines innate immunity with adaptive immunity by utilizing spatially distinct regions for agonist selection and antigen responsiveness. Nat Immunol 19:1352-1365 (2018). PubMed: 30420626
  • Tyc KM  et al. The Conserved Intron Binding Protein EMB-4 Plays Differential Roles in Germline Small RNA Pathways of C. elegans. Dev Cell 42:256-270.e6 (2017). PubMed: 28787592
  • Di Marco Barros R  et al. Epithelia Use Butyrophilin-like Molecules to Shape Organ-Specific ?d T Cell Compartments. Cell 167:203-218.e17 (2016). PubMed: 27641500
  • Crawford JR  et al. Plasma Levels of Endothelial Microparticles Bearing Monomeric C-reactive Protein are Increased in Peripheral Artery Disease. J Cardiovasc Transl Res 9:184-93 (2016). PubMed: 26891844

客户评价及客户问答

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Question

I am interested in the Mouse TCS Purification System. The instructions say that the the mouse antibody should be in 10 to 25 mL of tissue culture supernatant. Would it be possible to use the kit if the antibody is in a larger volume than 25 mL (but still less than 1.5 mg of antibody)?

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Answer

Thank you for your reply.

I have again talked to the lab, and they have said that 6ml of 10X Binding Buffer, there should be more than enough binding buffer for your experiments and so you do not need to worry about not having enough. As for pH, as long as it within normal ranges that allow cells to grow well, you should have no issues.

If there is anything else I can help you with, please let me know.

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Question

What is the elution buffer? I need to avoid amines and thiols for the subsequent conjugation.

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Answer

Although the formulation of the elution buffer is considered proprietary, the laboratory offers the following information:

The elution buffer does not contain any amines or thiols. It should be noted that the customer should follow the protocol and proceed to the neutralization step after the elution step. This is because the elution buffer is of low (acidic) pH. Following neutralization there should be no amines or thiols present in the eluate.

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Question

Hello,

I am interested in the Mouse TCS Purification System. The instructions say that the the mouse antibody should be in 10 to 25 mL of tissue culture supernatant. Would it be possible to use the kit if the antibody is in a larger volume than 25 mL (but still less than 1.5 mg of antibody)?

Read More
Answer

Thank you for contacting Abcam.

I have talked to the lab and they have told me that if the amount (mg) of antibody is indeed below the capacity stated, then this product can be used with larger sample volumes (within reason).

Please let me know if there is anything else I can help you with.

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