FITC荧光偶联试剂盒(Fast) - Lightning-Link®

• Fluorescence Microscopy

PubMed

Fluorescence Microscopy - FITC Conjugation Kit (Fast) - Lightning-Link® (AB188285)

Fluorescence Microscopy - FITC Conjugation Kit (Fast)- Lightning-Link.

Baranowski, Andreas, et al used FITC Conjugation Kit (Fast) - Lightning-Link^® (ab188285) as part of examining whether the bioactive coating of calcium phosphate cements (CPCs) with bone sialoprotein (BSP) results in increased bone formation. They used the kit to conjugate FITC to bone sialoprotein prior to the coating of a three-dimensional printed CPC scaffolds. After several washing steps, the remaining BSP (green) was visualized via microscope and the BSP coating was thus qualitatively evaluated.

Image from Baranowski, Andreas, et al., Materials, 11(11):2336, doi: 10.3390/ma11112336. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• Schematic Diagram

Supplier Data

Schematic Diagram - FITC Conjugation Kit (Fast) - Lightning-Link® (AB188285)

This illustration demonstrates a general procedure of how the Lightning-Link®(Fast) labeling technology enables the direct labeling of antibodies or proteins.

Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for just 15 minutes. Please see the ab188285 protocol booklet for more details.

Learn more about our Lightning-Link® conjugation kits here

• ICC

PubMed

Immunocytochemistry - FITC Conjugation Kit (Fast) - Lightning-Link® (AB188285)

Immunocytochemistry - FITC Conjugation Kit (Fast) - Lightning-Link.

Do, Danh C., et al used FITC Conjugation Kit (Fast) - Lightning-Link^® (ab188285) as part of examining Bla g 2 uptake by human basophils. They used the kit to conjugate FITC to cockroach allergen Bla g 2 for use in immunocytochemistry.
Human basophils were incubated with FITC conjugated Bla g 2 (FITC‐Bla g 2, green) for overnight at 37°C, washed, and analyzed by fluorescent microscopy. Nucleus was stained with the DAPI (blue).

Image from Do, Danh C., et al., Immunity, Inflammation and Disease; 5(4):386-399. doi: 10.1002/iid3.145. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• Flow Cyt

PubMed

Flow Cytometry - FITC Conjugation Kit (Fast) - Lightning-Link® (AB188285)

Albus, Alexandra, et al used FITC Conjugation Kit (Fast) - Lightning-Link^® (ab188285) as part of examining Naturally occurring autoantibodies (nAb)-Secreting B1 Cells. They used the kit to conjugate FITC to anti-α-Synuclein antibody for use in flow cytometry.
nAbs-secreting B1 cells are rare and highly specific. (A–F) Gating strategy to sort only CD20+ CD27+ CD43+ CD69− IgG+, and Aβ+ (E) /α-Syn+ (F) B cells. Percentages above each plot indicate the proportion of cells resulting from the previous gate. Arrows indicate single cells in gate R5. CD, cluster of differentiation; FSC, forward scatter; SSC, sideward scatter.

Image from Albus et al., Fron. Immunol., 10:2033; doi: 10.3389/fimmu.2019.02033. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• Flow Cyt

PubMed

Flow Cytometry - FITC Conjugation Kit (Fast) - Lightning-Link® (AB188285)

Flow Cytometry - FITC Conjugation Kit (Fast)- Lightning-Link.

Robinson, Andrew P., et al used FITC Conjugation Kit (Fast) - Lightning-Link^® (ab188285) as part of characterizing oligodendroglial populations. They used the kit to conjugate FITC to Rat anti-PDGFRalpha antibody, clone APA5, for use in flow cytometry.
SJL/J mice were immunized with PLP139–151 and scored daily for clinical disease. A cohort of SJL/J mice was sacrificed, and spinal cords were analyzed by flow cytometry (n = 5). (A) Cells were distinguished from debris by forward and side scatter then singlet cells were gated. Live cells were gated by dead cell exclusion, and CNS resident cells were identified as CD45− or CD45low. (B) Oligodendroglial cells were defined by double positive staining : A2B5+PDGFRα+ early OPCs, A2B5+NG2+ intermediate OPCs, NG2+O4+ late OPCs, O4+MOG+ pre-myelinating oligodendrocytes, and GALC+MOG+ mature oligodendrocytes.

Image from Robinson, Andrew P., et al., PloS one, 9(9):e107649. doi: 10.1371/journal.pone.0107649. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• LFA

PubMed

Lateral flow assay - FITC Conjugation Kit (Fast) - Lightning-Link® (AB188285)

Rey, Elizabeth G., Julia L. Finkelstein, and David Erickson used FITC Conjugation Kit (Fast) - Lightning-Link^® (ab188285) as part of examining folate concentration in human serum. They used the kit to conjugate FITC to FA-BSA conjugates for use in lateral flow assay.
Schematic of sample processing and lateral flow assay, and human serum results. (a) Combination of serum sample with high-pH buffer. (b) Heating of serum solution. (c) Addition of acidic buffer to lower pH. (d) Application of prepared serum solution to LFA. (e) Application of running buffer. (f) Addition of FITC conjugates to nitrocellulose membrane. Human serum results. (g) Mean T/C ratio versus folate concentration for 24 human serum samples. Error bars shown are standard deviation, n = 3. Dotted line shows four-parameter logistic curve fit. (h,i) Images of fluorescent signal from test strip for low and high folate concentration serum, respectively.

Image from Rey et al., PLoS One, 14(6):e0217403; doi: 10.1371/journal.pone.0217403. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• Conjugation

PubMed

Conjugation - FITC Conjugation Kit (Fast) - Lightning-Link® (AB188285)

Lydon et al. used ab188285 FITC Conjugation Kit to label mouse anti-sheep CD34 antibody with FITC.

Data shows a) Representative light-scatter (SSC-A) vs fluorescence (CD34-FITC) plot of CD34 expression on cells from ovine apheresis samples using two-colour flow cytometry. B) IgG control.

Image from Lydon H et al., BMC veterinary research., 14 (1) 47. Fig 5.; doi: 10.1186/s12917-018-1332-4. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/.

• Coag

PubMed

Coagulation - FITC Conjugation Kit (Fast) - Lightning-Link® (AB188285)

FITC Conjugation Kit labeling anti MUC1 SP antibodies FACS

Image from Kovjazin R et al., PLoS One, 9(1):e85400. Fig 3.; doi: 10.1371/journal.pone.0085400. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/