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Alexa Fluor® 700偶联试剂盒(Fast) - Lightning-Link® (ab269824)

  • Datasheet
  • SDS
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AlexaFluor 700 spectra

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    概述

    • 产品名称

      Alexa Fluor® 700偶联试剂盒(Fast) - Lightning-Link®
    • 产品概述

      Alexa Fluor® 700 Conjugation Kit / Alexa Fluor® 700 Labeling Kit ab269824 uses a simple and quick process for Alexa Fluor® 700 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.


      To conjugate an antibody to Alexa Fluor® 700 using this kit:
      - add modifier to antibody and incubate for 15 mins
      - add quencher and incubate for 5 mins
      The Alexa Fluor® 700 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


      Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to Alexa Fluor® 700.


      Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

    • 说明

      This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Alexa Fluor® 700 Labeling Kit. 337-0015 is the same as the 1 mg size. 337-0010 is the same as the 3 x 100 ug size. 337-0030 is the same as the 3 x 10 ug size.

      Amount and volume of antibody for conjugation to Alexa Fluor® 700

       Kit size

      Recommended 
      amount of antibody
      1 

      Maximum 
      amount of antibody

      Maximum antibody 
      volume2

      3 x 10 µg

      3 x 10 µg

      3 x 20 µg

      3 x 10 µL

      100 µg

      1 x 100 µg

      1 x 200 µg

      1 x 100 µL

      3 x 100 µg

      3 x 100 µg 

      3 x 200 µg

      3 x 100 µL

      1 mg

      1 x 1 mg

      1 x 2 mg

      1 x 1 mL

      1 Using the maximum amount of antibody may result in less labelling per antibody. 

      2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2mg/ml or < 0.5 mg/ml should be diluted /concentrated.

      Buffer Requirements for Conjugation

      Buffer should be pH 6.5-8.5.

      Compatible buffer constituents 
      If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

      50mM / 0.6% Tris1 0.1% BSA2 50% glycerol
      0.1% sodium azide PBS Potassium phosphate
      Sodium chloride HEPES Sucrose
      Sodium citrate EDTA Trehalose

      1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
      2 BSA can also interfere with the use of the conjugated antibody in tissue staining.

      Incompatible buffer constituents

      Thiomerosal Proclin Glycine
      Arginine Glutathione DTT

      If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

      Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

      Storing and handling conjugation kits

      Lyophilized Lightning-Link® components are hygroscopic.

      Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    性能

    • 存放说明

      Store at -20°C. Please refer to protocols.
    • 组件 1 mg 100 µg 3 x 10 µg 3 x 100 µg
      ab274052 - Alexa Fluor 700 1 x 1mg 1 x 100µg 3 x 10µg 3 x 100µg
      ab273994 - Modifier reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl
      ab273995 - Quencher reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl

    图片

    • AlexaFluor 700 spectra
      AlexaFluor 700 spectra

    实验方案

    • Protocol Booklet
    • Protocol Booklet

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (2)

    发表研究结果有使用 ab269824?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab269824 被引用在 2 文献中.

    • Omatsu M  et al. THBS1-producing tumor-infiltrating monocyte-like cells contribute to immunosuppression and metastasis in colorectal cancer. Nat Commun 14:5534 (2023). PubMed: 37749092
    • Gregoire C  et al. Viral infection engenders bona fide and bystander subsets of lung-resident memory B cells through a permissive mechanism. Immunity 55:1216-1233.e9 (2022). PubMed: 35768001

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