DNA Library准备试剂盒(For Illumina®) (ab185903)
Key features and details
- Assay time: 2 hr 30 min
- Sample type: DNA
概述
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产品名称
DNA Library准备试剂盒(For Illumina®) -
样品类型
DNA -
检测时间
2h 30m -
产品概述
The DNA Library Preparation Kit (For Illumina®) (ab185903) is a complete set of optimized reagents to carry out a successful DNA library preparation. The kit is suitable for preparing a DNA library for next generation sequencing applications using an Illumina sequencer, which includes genomic DNA-seq, ChIP-seq, MeDIP/hMeDIP-seq, bisulfite-seq, and targeted re-sequencing. The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be constructed quickly with reduced bias.
Starting Materials
Starting materials can include fragmented dsDNA isolated from various tissue or cell samples, dsDNA enriched from ChIP reactions, MeDIP/hMeDIP reaction, or exon capture. DNA should be relatively free of RNA since large fractions of RNA will impair end repair and dA tailing, resulting in reduced ligation capabilities. Input amount of DNA can be from 5 ng to 1 µg. For optimal preparation, the input amount should be 100 ng to 200 ng. For amplification-free, 500 ng or more is needed.Illumina® is a registered trademark of Illumina, Inc.
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说明
DNA library preparation is a critical step for next generation sequencing (NGS). To generate accurate sequencing data for NGS, the prepared library DNA should be sufficient in yield and of high quality. Also, as NGS technology is continuously improving, DNA library preparation is required to be optimized accordingly. Most of the currently used methods are unfortunately time-consuming, expensive, and inconvenient. Some of the methods are relatively quick by combining end repair and dA tailing or even ligation in one-step, but have been shown to generate significant G tailing or form concatmers at the ligation step or have high insertion bias. These side reactions eventually result in the prepared DNA library being less efficient and inaccurate. An ideal DNA library preparation method should be balanced in speed, convenience, small sample-suitability, cost-effectiveness, and accuracy.
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经测试应用
适用于: ChIP-sequencingmore details
性能
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存放说明
Please refer to protocols. -
组件 12 tests 24 tests 10X dA-Tailing Buffer 1 x 40µl 1 x 80µl 10X End Repair Buffer 1 x 40µl 1 x 80µl 2X HiFi PCR Master Mix 1 x 160µl 1 x 320µl 2X Ligation Buffer 1 x 250µl 1 x 500µl Adaptors (50 μM) 1 x 15µl 1 x 30µl Elution Buffer 1 x 1ml 1 x 2ml End Repair Enzyme Mix 1 x 25µl 1 x 50µl Klenow Fragment (3’-5’ exo-) 1 x 15µl 1 x 30µl MQ Binding Beads 1 x 1.6ml 1 x 3.2ml Primer I (10 μM) 1 x 15µl 1 x 30µl Primer U (10 μM) 1 x 15µl 1 x 30µl T4 DNA Ligase 1 x 15µl 1 x 30µl -
研究领域
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab185903于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ChIP-sequencing |
Use at an assay dependent concentration.
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说明 |
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ChIP-sequencing
Use at an assay dependent concentration. |
图片
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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