PPAR alpha Transcription Factor Assay试剂盒(ab133107)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Nuclear Extracts, Suspension cells
概述
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产品名称
PPAR alpha Transcription Factor Assay试剂盒 -
检测方法
Colorimetric -
样品类型
Adherent cells, Suspension cells, Nuclear Extracts -
检测类型
Semi-quantitative -
种属反应性
与反应: Mouse, Rat, Human -
产品概述
Abcam's PPAR alpha Transcription Factor Assay Kit (ab133107) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR alpha is detected by addition of specific primary antibody directed against PPAR alpha. A secondary antibody conjugated to HRP is added to provide a sensitive colorometric readout at 450 nm. PPAR alpha Transcription Factor Assay will not cross-react with PPAR gamma or PPAR delta.
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说明
Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.
PPAR alpha is predominantly expressed in liver, heart and kidneys. Upon activation by ligands such as free fatty acids, lipids and other small molecules, PPAR alpha forms a heterodimeric complex with RXR alpha, and this complex will bind to response elements allowing transcription of target genes involved in lipid metabolism.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 96 tests 96-Well Plate Cover 1 unit Polysorbate 20 1 vial Transcription Factor Antibody Binding Buffer (10X) 1 x 3ml Transcription Factor Binding Assay Buffer (4X) 1 x 3ml Transcription Factor Developing Solution 1 x 12ml Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 x 100µl Transcription Factor PPAR 96-Well Strip Plate 1 unit Transcription Factor PPAR alpha Positive Control 1 vial Transcription Factor PPAR alpha Primary Antibody 1 vial Transcription Factor PPAR Competitor dsDNA 1 vial Transcription Factor Reagent A 1 x 120µl Transcription Factor Stop Solution 1 x 12ml Wash Buffer Concentrate (400X) 1 x 5ml -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
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功能
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. -
组织特异性
Skeletal muscle, liver, heart and kidney. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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别名
- hPPAR
- MGC2237
- MGC2452
see all -
数据库链接
- Entrez Gene: 5465 Human
- Entrez Gene: 19013 Mouse
- Entrez Gene: 25747 Rat
- Omim: 170998 Human
- SwissProt: Q07869 Human
- SwissProt: P23204 Mouse
- SwissProt: P37230 Rat
- Unigene: 103110 Human
see all
相关产品
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sELISA pair antibody
图片
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Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR alpha DNA-binding activity using ab133107.
Panel B: PPAR alpha DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab133107 被引用在 5 文献中.
- Watts AJ et al. Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus. Front Physiol 11:615025 (2020). PubMed: 33408645
- Ghosh S et al. Linoleic acid in diets of mice increases total endocannabinoid levels in bowel and liver: modification by dietary glucose. Obes Sci Pract 5:383-394 (2019). PubMed: 31452923
- Qi K et al. Tongxinluo attenuates reperfusion injury in diabetic hearts by angiopoietin-like 4-mediated protection of endothelial barrier integrity via PPAR-a pathway. PLoS One 13:e0198403 (2018). PubMed: 29912977
- Ren T et al. The Combination of Blueberry Juice and Probiotics Ameliorate Non-Alcoholic Steatohepatitis (NASH) by Affecting SREBP-1c/PNPLA-3 Pathway via PPAR-a. Nutrients 9:N/A (2017). PubMed: 28264426
- Bakshi MV et al. Total body exposure to low-dose ionizing radiation induces long-term alterations to the liver proteome of neonatally exposed mice. J Proteome Res 14:366-73 (2015). PubMed: 25299163