WI-38全细胞裂解物(ab3960)
概述
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产品名称
WI-38全细胞裂解物 -
常规说明
Cell line: WI-38 (Human lung fibroblast, normal).
Growth media: Minimum essential medium (Eagle) with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, and 10% FBS.WI-38 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Constituents: 12.5% Glycerol (glycerin, glycerine), 1% Sodium lauryl sulfate, 0.01% Bromophenol blue, 0.79% Tris HCl, 0.77% DTT -
Concentration information loading...
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裂解物说明
WI-38 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. -
研究领域
应用
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution. Ready to load; 10 - 20 µg/lane is recommended for mini gel.
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说明 |
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WB
Use at an assay dependent dilution. Ready to load; 10 - 20 µg/lane is recommended for mini gel. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab3960 被引用在 2 文献中.
- Taglialegna A et al. The biofilm-associated surface protein Esp of Enterococcus faecalis forms amyloid-like fibers. NPJ Biofilms Microbiomes 6:15 (2020). PubMed: 32221298
- Weitzel LR et al. Discovery and verification of protein differences between Er positive/Her2/neu negative breast tumor tissue and matched adjacent normal breast tissue. Breast Cancer Res Treat 124:297-305 (2010). PubMed: 20087651