SW 1353全细胞裂解物(ab7915)
概述
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产品名称
SW 1353全细胞裂解物 -
常规说明
Cell line: SW1353 (Human humerus chondrosarcoma, fibroblast-like).
Growth media: DMEM and 10% FBS (Fetal bovine serum).SW1353 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Constituent: 100% SDS Sample Buffer -
Concentration information loading... -
裂解物说明
SW1353 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. -
研究领域
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背景
The SW1353 chondrosarcoma cell line is considered to be a model for studies of the transcription factor Sox9, an important determinant of chondrocyte differentiation, including the regulation of type II collagen (Col2) and aggrecan gene expression. Cells are used to study mechanisms of joint inflammation and cartilage destruction.
应用
| 应用 | Ab评论 | 说明 |
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| WB |
Use at an assay dependent concentration. SW1353 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
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| 说明 |
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WB
Use at an assay dependent concentration. SW1353 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab7915 被引用在 1 文献中.
- Kinnavane L et al. Collateral Projections Innervate the Mammillary Bodies and Retrosplenial Cortex: A New Category of Hippocampal Cells. eNeuro 5:N/A (2018). PubMed: 29527569