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AB30124

Raji全细胞裂解物

Raji whole cell lysate

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Raji whole cell lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
4 Images
Western blot - Raji whole cell lysate (AB30124)
  • WB

Project

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-SOCS2 antibody (<a href='/products/primary-antibodies/socs2-antibody-ab66733'>ab66733</a>) at 1 µg/mL

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Western blot - SW480 whole cell lysate (<a href='/products/cell-lysates/sw480-whole-cell-lysate-ab3957'>ab3957</a>) at 10 µg

Lane 4:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Lane 5:

Western blot - Mouse thymus tissue lysate - total protein (<a href='/products/tissue-lysates/mouse-thymus-tissue-lysate-total-protein-ab29285'>ab29285</a>) at 10 µg

Lane 6:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Western blot - Raji whole cell lysate (AB30124)
  • WB

Project

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-BRD1/BRL antibody (<a href='/products/primary-antibodies/brd1-brl-antibody-ab71877'>ab71877</a>) at 1 µg/mL

Lane 1:

TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

Lane 2:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Observed band size: 150 kDa

false

Western blot - Raji whole cell lysate (AB30124)
  • WB

Project

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-HYAL2 antibody (<a href='/products/primary-antibodies/hyal2-antibody-ab68608'>ab68608</a>) at 1 µg/mL

Lane 1:

Liver (Rat) Tissue Lysate at 10 µg

Lane 2:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 3:

JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Human thymus tissue lysate - total protein (<a href='/products/unavailable/human-thymus-tissue-lysate-total-protein-ab30146'>ab30146</a>) at 10 µg

Lane 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Lane 8:

Western blot - SW480 whole cell lysate (<a href='/products/cell-lysates/sw480-whole-cell-lysate-ab3957'>ab3957</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 54 kDa

Observed band size: 40 kDa,50 kDa,54 kDa,58 kDa

true

Western blot - Raji whole cell lysate (AB30124)
  • WB

Project

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-ATG3 antibody (<a href='/products/primary-antibodies/atg3-antibody-ab66287'>ab66287</a>) at 1 µg/mL

Lane 1:

TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Lane 3:

JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 35 kDa

Observed band size: 36 kDa,72 kDa

false

关键信息

细胞类型

Raji

种属

Human

组织

Lymphatic

形式

Liquid

form

疾病

Lymphoma

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Samples need to boiled for 5 minutes and put on ice before use.</p>" } } }

产品详情

Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Allow to warm to room temp and agitate gently before aliquoting

细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Suspension

性别

Male

产品实验方案

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂,为您科研的每一步提供支持。若我们的产品未能达到预期性能,我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

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