人ZNF622 (ZPR9) knockout HeLa cell裂解物
Human ZNF622 (ZPR9) knockout HeLa cell lysate
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ZNF622 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon1 and 1 bp deletion in exon1 and 1 bp insertion in exon1.
查看别名
ZNF622, Cytoplasmic 60S subunit biogenesis factor ZNF622, Zinc finger protein 622, Zinc finger-like protein 9, ZPR9
- Sanger seq
Unknown
Sanger Sequencing - Human ZNF622 (ZPR9) knockout HeLa cell lysate (AB258770)
Allele-3 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human ZNF622 (ZPR9) knockout HeLa cell lysate (AB258770)
Allele-2 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human ZNF622 (ZPR9) knockout HeLa cell lysate (AB258770)
Allele-1 : 16 bp deletion in exon1
搭配使用产品
产品详情
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
规格
性能和储存信息
基因名称
基因编辑类型
基因编辑方法
敲除验证
运输条件
推荐的短期储存条件
推荐的长期储存条件
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ZPR9 plays a role in managing cellular processes that include transcriptional regulation and cellular signaling. It often associates with other proteins to form complexes that modulate gene expression in response to external stimuli. Research has shown that ZPR9 interacts with components of the transcription machinery influencing numerous biological functions that encompass immune system modulation and cell cycle progression.
Pathways
ZPR9 contributes significantly to the MAPK signaling pathway and NF-kB pathway. In the MAPK pathway it connects with proteins such as ERK1/2 impacting cellular responses like proliferation and differentiation. In the NF-kB pathway ZPR9 has links with protein p65 contributing to the regulation of immune responses and inflammation. These connections indicate its integration in key signaling networks vital for cellular homeostasis and immune regulation.
质量控制
STR 分析
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
细胞培养
生物安全等级
EU: 2 US: 2
贴壁/悬浮
Adherent
性别
Female
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com