AB258246

人TRIM24 knockout HeLa cell裂解物

Name: 人TRIM24 knockout HeLa cell lysate (ab258246)| Abcam中文官网
Brand: Abcam Limited
SKU: AB258246
Price: 5956 CNY
Availability: InStock

Human TRIM24 knockout HeLa cell lysate

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TRIM24 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.

查看别名

E3 ubiquitin-protein ligase Trim24, PTC6, RING finger protein 82, RNF82, TF1A, TIF1, TIF1-alpha, TIF1A_HUMAN, Transcription intermediary factor 1-alpha, Transcriptional intermediary factor 1, Transcriptional intermediary factor 1 alpha, Tripartite motif containing 24, Tripartite motif-containing protein 24, hTIF1

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Western blot - Human TRIM24 knockout HeLa cell lysate (AB258246)

Lane 1 : Wild-type HeLa cell lysate (20μg)

Lane 2 : TRIM24 knockout HeLa cell lysate (20μg)

Lane 3 : Hap1 cell lysate (20μg)

Lane 4 : A549 cell lysate (20μg)

Lanes 1- 4 : Merged signal (red and green). Green - ab256491 observed at 140 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab256491 Anti-TRIM24 antibody [EPR22825-2] was shown to specifically react with Tripartite Motif Containing 24 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264963 (knockout cell lysate ab258246) lane below 140kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and Tripartite Motif Containing 24 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab256491 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRIM24 antibody [EPR22825-2] (<a href='/products/primary-antibodies/trim24-antibody-epr22825-2-ab256491'>ab256491</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TRIM24 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TRIM24 knockout HeLa cell lysate (ab258246)

Lane 2:

Western blot - Human TRIM24 knockout HeLa cell line (<a href='/products/cell-lines/human-trim24-knockout-hela-cell-line-ab264963'>ab264963</a>)

Lane 3:

Hap1 cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Predicted band size: 116 kDa

Observed band size: 140 kDa

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Sanger Sequencing - Human TRIM24 knockout HeLa cell lysate (AB258246)

Sanger seq

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Sanger Sequencing - Human TRIM24 knockout HeLa cell lysate (AB258246)

Allele-2 : Insertion of the selection cassette in exon 1

Sanger seq

Unknown

Sanger Sequencing - Human TRIM24 knockout HeLa cell lysate (AB258246)

Allele-1 : 2 bp insertion in exon 1

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

敲除验证

Sanger Sequencing

突变描述

Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.

疾病

Adenocarcinoma

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.</p>" } } }

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产品详情

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258246-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human TRIM24 knockout HeLa cell lysate", "number":"AB258246-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258246-CMP01", "productcode":"" } ] } } }

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性能和储存信息

基因名称

TRIM24

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Sanger Sequencing

运输条件

Ambient - Can Ship with Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRIM24 also known as transcription intermediary factor 1-alpha (TIF1α) is a protein that acts mechanically as a transcriptional regulator. It has a molecular mass of approximately 140 kDa. This protein contains several domains including a tripartite motif (the eponymous TRIM) which consists of a RING domain B-box domains and a coiled-coil region. TRIM24 interacts with nuclear receptors and is expressed in various tissues but shows higher expression in the liver and lungs. It also plays a role in bridging the interaction between chromatin and transcription factors.

Biological function summary

TRIM24 is involved in transcriptional regulation by influencing gene expression. It acts as a co-regulator being part of large protein complexes. This protein interacts directly with histone tails to read histone marks and release transcriptional repression. TRIM24 also plays a role in ubiquitination and subsequent proteasomal degradation of specific proteins suggesting its involvement in maintaining protein homeostasis. Additionally TRIM24 has been shown to interact with p53 influencing cell cycle regulation and apoptosis.

Pathways

TRIM24 is involved in the regulation of the retinoic acid and vitamin D signaling pathways. It modulates the transcriptional activity of nuclear receptors through these pathways influencing cell proliferation and differentiation processes. TRIM24 does so by interacting with other proteins such as retinoid X receptor (RXR) and estrogen receptor (ER) establishing important crosstalk between signaling cascades critical for cell fate decisions.

TRIM24 has been implicated in the development of certain cancers such as breast cancer and liver cancer. In these contexts it functions as an oncogene promoting tumorigenesis through aberrant transcriptional regulation. TRIM24 can interact with other proteins associated with oncogenesis including p53 modulating its tumor suppressor functions. Furthermore deregulation of TRIM24 activity or expression levels has been correlated with inflammatory disorders highlighting its relevance in both cancer and inflammation-related conditions.

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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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Abcam Product Promise

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人TRIM24 knockout HeLa cell裂解物

Human TRIM24 knockout HeLa cell lysate

Western blot - Human TRIM24 knockout HeLa cell lysate (AB258246)

Sanger Sequencing - Human TRIM24 knockout HeLa cell lysate (AB258246)

Sanger Sequencing - Human TRIM24 knockout HeLa cell lysate (AB258246)