人TMEM106B knockout A549 cell裂解物

• NGS

Supplier Data

Next Generation Sequencing - Human TMEM106B knockout A549 cell lysate (AB273769)

Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift : 100%

• WB

Lab

Western blot - Human TMEM106B knockout A549 cell lysate (AB273769)

All lanes:

Western blot - Anti-TMEM106B antibody [EPR30569-44] - C-terminal (<a href='/products/primary-antibodies/tmem106b-antibody-epr30569-44-c-terminal-ab325607'>ab325607</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TMEM106B knockout A549 cell lysate (ab273769) at 20 µg

Lane 3:

AsPC-1 (human Pancreas epithelial cell) whole cell lysate at 20 µg

Lane 4:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 6:

3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa,40 kDa,36 kDa

true

Exposure time: 103s

• WB

Lab

Western blot - Human TMEM106B knockout A549 cell lysate (AB273769)

Western blot : Anti-TMEM106B antibody staining at 0.1 µg/ml, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, the antibody was shown to bind specifically to TMEM106B. A band was observed at 45 kDa in wild-type A549 cell lysates with no signal observed at this size in TMEM106B knockout cell line ab273711 (knockout cell lysate ab273769). To generate this image, wild-type and TMEM106B knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Anti-TMEM106B antibody staining at 0.1 µg/mL

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human TMEM106B knockout A549 cell lysate (ab273769) at 20 µg

Lane 3:

PANC-1 cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

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