AB261685

人TGM2 (Transglutaminase 2) knockout A549 cell裂解物

Name: 人TGM2 (Transglutaminase 2) knockout A549 cell lysate (ab261685)| Abcam中文官网
Brand: Abcam Limited
SKU: AB261685
Price: 6405 CNY
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Human TGM2 (Transglutaminase 2) knockout A549 cell lysate

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TGM2 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion 8 bp deletion Frameshift = 97%.

查看别名

ALPHA SUBUNIT, C polypeptide, EC 2.3.2.13, G alpha h, GNAH, GNAH G PROTEIN, G[a]h, Gh CLASS G ALPHA h, H POLYPEPTIDE, HEL-S-45, Protein-glutamine gamma-glutamyltransferase 2, TG 2, TG(C), TGC, TGC GUANINE NUCLEOTIDE BINDING PROTEIN, TGM2_HUMAN, TGase C, TGase H, TGase-2, TgaseII, Tissue transglutaminase, Transglutaminase 2 C polypeptide, Transglutaminase C, Transglutaminase H, Transglutaminase-2, epididymis secretory protein Li 45, tTG, tTGas

3 Images

Lab

Western blot - Human TGM2 (Transglutaminase 2) knockout A549 cell lysate (AB261685)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 2 : TGM2 knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab2386 observed at 77 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab2386 was shown to recognize in wild-type A549 cells as signal was lost at the expected MW in TGM2 knockout cell line ab261876 (knockout cell lysate ab261685). Additional cross-reactive bands were observed in the wild-type and knockout samples. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab2386 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Transglutaminase 2 antibody [CUB 7402] (<a href='/products/primary-antibodies/transglutaminase-2-antibody-cub-7402-ab2386'>ab2386</a>) at 1 µg/mL

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TGM2 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TGM2 (Transglutaminase 2) knockout A549 cell line (<a href='/products/cell-lines/human-tgm2-transglutaminase-2-knockout-a549-cell-line-ab261876'>ab261876</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Predicted band size: 77 kDa

Observed band size: 77 kDa

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Lab

Western blot - Human TGM2 (Transglutaminase 2) knockout A549 cell lysate (AB261685)

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lane 2 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate 20 ug

Lane 3 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 4 : TGM2 knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab64771 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab64771 was shown to specifically react with in wild-type A549 cells as signal was lost in TGM2 knockout cell line ab261876 (knockout cell lysate ab261685). Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. ab64771 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Anti-Transglutaminase 2 antibody (<a href='/products/unavailable/transglutaminase-2-antibody-ab64771'>ab64771</a>) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Lane 3:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

TGM2 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Western blot - Human TGM2 (Transglutaminase 2) knockout A549 cell line (<a href='/products/cell-lines/human-tgm2-transglutaminase-2-knockout-a549-cell-line-ab261876'>ab261876</a>)

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Supplier Data

Next Generation Sequencing - Human TGM2 (Transglutaminase 2) knockout A549 cell lysate (AB261685)

Knockout achieved by CRISPR/Cas9; X = 1 bp insertion, 8 bp deletion; Frameshift = 97%

关键信息

细胞类型

A549

种属

Human

组织

Lung

敲除验证

Next Generation Sequencing,Western blot

突变描述

Knockout achieved by CRISPR/Cas9 X = 1 bp insertion 8 bp deletion Frameshift = 97%

疾病

Carcinoma

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab261685-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human TGM2 knockout A549 cell lysate", "number":"AB261685-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type A549 cell lysate", "number":"AB261685-CMP01", "productcode":"" } ] } } }

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性能和储存信息

基因名称

TGM2

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Next Generation Sequencing, Western blot

运输条件

Ambient - Can Ship with Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Transglutaminase 2 also known as TG2 or tissue transglutaminase is an enzyme with a mass of approximately 74 kDa. It catalyzes the formation of covalent bonds by crosslinking glutamine residues and lysine residues in proteins. This enzyme is widely expressed in various tissues including the liver heart and lungs. It can act as a GTPase a kinase and a protein disulfide isomerase highlighting its multifunctional nature. Additionally transglutaminase 2 associates with cell membranes cytoskeleton and extracellular matrix indicating its versatile involvement in cellular processes.

Biological function summary

Transglutaminase 2 plays roles in cell adhesion wound healing and the immune response. It mediates extracellular matrix stabilization through crosslinking structural proteins which is critical for tissue repair. The enzyme does not typically form a stable part of a large complex but interacts with fibronectin as a binding partner highlighting its role in cellular adhesion and migration. The enzymatic activity of transglutaminase 2 helps maintain tissue integrity by modifying extracellular and intracellular proteins.

Pathways

Transglutaminase 2 contributes significantly to the integrin-mediated signaling pathway and the apoptosis pathway. It assists in the modulation of cell-matrix interactions by crosslinking matrix proteins which is important for integrin signaling. During apoptosis transglutaminase 2 promotes cellular processes that lead to cell death via its involvement in nuclear condensation and DNA fragmentation. In these pathways proteins such as fibronectin and integrins interact with transglutaminase 2 facilitating its regulatory functions.

Transglutaminase 2 is associated with celiac disease and neurodegenerative disorders like Huntington's disease. Its autoantigenic properties make it a target in celiac disease where antibodies against transglutaminase 2 play a role in the pathology by affecting intestinal tissue. In Huntington's disease transglutaminase 2 contributes to the formation of protein aggregates which are toxic to neurons. This enzyme's interactions with proteins such as gliadin in celiac disease and huntingtin in Huntington's disease are central to its pathogenic mechanism.

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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 1 US: 1

贴壁/悬浮

Adherent

性别

Male

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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Abcam Product Promise

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人TGM2 (Transglutaminase 2) knockout A549 cell裂解物

Human TGM2 (Transglutaminase 2) knockout A549 cell lysate

Western blot - Human TGM2 (Transglutaminase 2) knockout A549 cell lysate (AB261685)

Western blot - Human TGM2 (Transglutaminase 2) knockout A549 cell lysate (AB261685)

Next Generation Sequencing - Human TGM2 (Transglutaminase 2) knockout A549 cell lysate (AB261685)