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AB257726

人TAX1BP1 (TRAF6BP) knockout HEK-293T cell裂解物

Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate

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TAX1BP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 5.

查看别名

1200003J11Rik, 1700069J21Rik, AA930106, Aa1076, CALCOCO 3, D6Ertd404e, D6Ertd772e, Human T cell leukemia virus type 1-binding protein, Liver regeneration-related protein LRRG004, MGC94031, PRO0105, T6BP, TAX1BP1, TAX1BP1 protein, TAXB1_HUMAN, TRAF6 binding protein T6BP, TRAF6-binding protein, TRAF6-interacting protein, TXBP 151, Tax1 (human T cell leukemia virus type I) binding protein, Tax1 (human T cell leukemia virus type I) binding protein 1, Tax1 binding protein, Tax1-binding protein 1, Tax1-binding protein 1 homolog, tax1bp1b, wu:fc20c12, wu:fc56e11, zgc:73219, zgc:77129

2 Images
Western blot - Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate (AB257726)
  • WB

Lab

Western blot - Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate (AB257726)

Anti-TAX1BP1 antibody [EPR13287(B)] (ab176572) staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab176572 was shown to bind specifically to TAX1BP1. A band was observed at 95 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TAX1BP1 knockout cell line. To generate this image, wild-type and TAX1BP1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (<a href='/products/primary-antibodies/traf6bp-tax1bp1-antibody-epr13287b-c-terminal-ab176572'>ab176572</a>) at 1/2000 dilution

Lanes 1 - 2:

Western blot at 20 µg

Lane 2:

Western blot - Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate (ab257726)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 91 kDa

Observed band size: 95 kDa

false

Sanger Sequencing - Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate (AB257726)
  • Sanger seq

Unknown

Sanger Sequencing - Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate (AB257726)

Homozygous : 13 bp deletion in exon 5

关键信息

细胞类型

HEK-293T

种属

Human

组织

Kidney

敲除验证

Sanger Sequencing

突变描述

Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 5.

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称
TAX1BP1
基因编辑类型
Knockout
基因编辑方法
CRISPR technology
敲除验证
Sanger Sequencing
合子性
Homozygous
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRAF6BP also known as TAX1BP1 is a cytoplasmic protein that participates in important signaling pathways. It has an approximately 75 kDa molecular weight and mainly expresses in immune-related cells including macrophages and lymphocytes. TRAF6BP works as an adaptor protein binding to TRAF6 and regulating its ubiquitin ligase activity which is key in transmitting signals inside the cell.
Biological function summary

TRAF6BP/TAX1BP1 regulates the immune response through its interaction with TRAF6. It plays a part in the formation of a protein complex that influences the balance between pro-inflammatory and anti-inflammatory signals. TRAF6BP affects autophagy an important process for cellular homeostasis and pathogen elimination. TRAF6BP also manages cell survival and apoptosis through its role in signaling cascades.

Pathways

TRAF6BP/TAX1BP1 is heavily involved in the NF-kB and JNK signaling pathways which are pivotal for immune and inflammatory responses. TRAF6BP interacts with proteins like TRAF6 and TAK1 within these pathways to mediate downstream effects that lead to cytokine production and stress responses. These pathways play large roles in maintaining the immune system's balance.

TRAF6BP/TAX1BP1 has connections with inflammatory conditions and certain cancers. It associates with chronic inflammatory diseases due to its role in regulating cytokine production. Changes in TRAF6BP expression or function can lead to unregulated inflammation which is characteristic of rheumatoid arthritis. Furthermore TRAF6BP's regulatory effect on the NF-kB pathway links it to cancer development particularly in cases where overactive NF-kB signaling promotes tumor growth. Its interaction with TRAF6 is critical in these disease contexts.
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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

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搭配使用产品

Primary Antibodies

AB176572

Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal

RabMAb|Recombinant|KO Validated

Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (AB176572)

  • 0
  • 0
Primary Antibodies

AB4448

Anti-Pericentrin antibody - Centrosome Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (AB4448)

  • 5
  • 35
Primary Antibodies

AB43840

Anti-Cenexin1/ODF2 antibody

Western blot - Anti-Cenexin1/ODF2 antibody (AB43840)

  • 4
  • 4
Primary Antibodies

AB133315

Anti-ARPC2 antibody [EPR8533]

RabMAb|Recombinant

Western blot - Anti-ARPC2 antibody [EPR8533] (AB133315)

  • 5
  • 3

Abcam Product Promise

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