人TAL1 knockout Jurkat cell裂解物(ab273816)
概述
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产品名称
人TAL1 knockout Jurkat cell裂解物 -
产品概述
Knockout cell lysate achieved by CRISPR/Cas9.
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Parental Cell Line
Jurkat -
Organism
Human -
Mutation description
Knockout achieved by CRISPR/Cas9; X = 2 bp insertion; Frameshift: 100% -
Passage number
<20 -
Knockout validation
Next Generation Sequencing (NGS) -
Reconstitution notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
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说明
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
See here for more information on knockout cell lysates.Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
性能
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存放说明
Store at -80°C. Please refer to protocols. -
组件 1 kit ab280666 - Human TAL1 knockout Jurkat cell lysate 1 x 100µg ab269598 - Human wild-type Jurkat cell lysate 1 x 100µg -
研究领域
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Cell type
T cell lymphoblast-like -
Disease
Non-Hodgkin Lymphoma -
Gender
Male
靶标
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功能
Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation. -
组织特异性
Leukemic stem cell. -
疾病相关
Note=A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). Translocation t(1;14)(p32;q11) with T-cell receptor alpha chain (TCRA) genes. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
结构域
The helix-loop-helix domain is necessary and sufficient for the interaction with DRG1. -
翻译后修饰
Phosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia.
Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells. -
细胞定位
Nucleus. - Information by UniProt
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别名
- bHLHa17
- Class A basic helix-loop-helix protein 17
- OTTHUMP00000009563
see all
相关产品
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KO cell lines
图片
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Next Generation Sequencing - Human TAL1 knockout Jurkat cell lysate (ab273816)Knockout achieved by CRISPR/Cas9; X = 2 bp insertion; Frameshift: 100%
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab273816 尚未被引用在任何文献中。