AB257688

人SMARCB1 (SNF5) knockout HEK-293T cell裂解物

Name: 人SMARCB1 (SNF5) knockout HEK-293T cell lysate (ab257688)| Abcam中文官网
Brand: Abcam Limited
SKU: AB257688
Price: 6206 CNY
Availability: InStock

Human SMARCB1 (SNF5) knockout HEK-293T cell lysate

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SMARCB1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon2 and 1 bp insertion in exon2.

查看别名

BAF47, BRG1-associated factor 47, INI1, Integrase interactor 1 protein, Malignant rhabdoid tumor suppressor, RDT, RTPS1, SMARCB1, SNF5 homolog, SNF5L1, SNF5_HUMAN, SWI/SNF complex component SNF5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, SWI10, Sfh1p, Snr1, Sucrose nonfermenting yeast homolog like 1, TYE4, Transcription factor TYE4, Transcription regulatory protein SNF5, hSNF5

4 Images

Lab

Western blot - Human SMARCB1 (SNF5) knockout HEK-293T cell lysate (AB257688)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : SMARCB1 knockout HEK293T cell lysate (20 ug)
Lane 3 : Daudi cell lysate (20 ug)
Lane 4 : HeLa cell lysate (20 ug)

ab192864 was shown to specifically react with SNF5/SMARCB1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267269 (knockout cell lysate ab257688) was used. Wild-type and SNF5/SMARCB1 knockout samples were subjected to SDS-PAGE. ab192864 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014-77] (<a href='/products/primary-antibodies/snf5-smarcb1-antibody-epr12014-77-ab192864'>ab192864</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

SMARCB1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human SMARCB1 (SNF5) knockout HEK-293T cell line (<a href='/products/cell-lines/human-smarcb1-snf5-knockout-hek-293t-cell-line-ab267269'>ab267269</a>)

Lane 3:

Daudi cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 44 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Human SMARCB1 (SNF5) knockout HEK-293T cell lysate (AB257688)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : SMARCB1 knockout HEK293T cell lysate (20 ug)
Lane 3 : Daudi cell lysate (20 ug)
Lane 4 : HeLa cell lysate (20 ug)

ab126734 was shown to specifically react with SNF5/SMARCB1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267269 (knockout cell lysate ab257688) was used. Wild-type and SNF5/SMARCB1 knockout samples were subjected to SDS-PAGE. ab126734 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (<a href='/products/primary-antibodies/snf5-smarcb1-antibody-epr6966-ab126734'>ab126734</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

SMARCB1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human SMARCB1 (SNF5) knockout HEK-293T cell line (<a href='/products/cell-lines/human-smarcb1-snf5-knockout-hek-293t-cell-line-ab267269'>ab267269</a>)

Lane 3:

Daudi cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 44 kDa,84 kDa

Observed band size: 50 kDa,90 kDa

false

Sanger Sequencing - Human SMARCB1 (SNF5) knockout HEK-293T cell lysate (AB257688)

Sanger seq

Unknown

Sanger Sequencing - Human SMARCB1 (SNF5) knockout HEK-293T cell lysate (AB257688)

Allele-2 : 1 bp insertion in exon2

Sanger seq

Unknown

Sanger Sequencing - Human SMARCB1 (SNF5) knockout HEK-293T cell lysate (AB257688)

Allele-1 : 16 bp deletion in exon2

关键信息

细胞类型

HEK-293T

种属

Human

组织

Kidney

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon2 and 1 bp insertion in exon2.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257688-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human SMARCB1 knockout HEK293T cell lysate", "number":"AB257688-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257688-CMP02", "productcode":"" } ] } } }

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性能和储存信息

基因名称

SMARCB1

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Sanger Sequencing, Western blot

运输条件

Ambient - Can Ship with Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SNF5/SMARCB1 also known as INI1 or BAF47 is a member of the SWI/SNF chromatin remodeling complex. It acts in modifying chromatin structure to regulate gene expression. SNF5/SMARCB1 possesses a mass of approximately 47 kDa. The protein expresses ubiquitously in various tissues with notable presence in the nucleus. Through its mechanical activity in chromatin remodeling SNF5/SMARCB1 facilitates transcriptional regulation and chromosomal stability.

Biological function summary

SNF5/SMARCB1 acts as an integral component of the SWI/SNF complex. This complex functions in altering nucleosome positioning on DNA impacting gene expression linked to cell cycle regulation differentiation and DNA repair. By working within this complex SNF5/SMARCB1 helps in the activation or repression of specific gene sets based on cellular requirements displaying an essential role in maintaining normal cellular function. The interplay of SNF5/SMARCB1 with other chromatin-modifying enzymes extends its influence across various genetic pathways.

Pathways

The involvement of SNF5/SMARCB1 in the cell cycle and transcription regulation pathways is acknowledged. It participates intimately in the p53 signaling pathway which is critical for cell cycle checkpoints and apoptotic responses. SNF5/SMARCB1 engages with other proteins such as p53 and RB1 coordinating to maintain genomic integrity and proper transcriptional programming. These pathways spotlight the protein's influence in important cell fate decisions.

SNF5/SMARCB1 mutations or deletions associate closely with malignant rhabdoid tumors an aggressive pediatric cancer and schwannomatosis a disorder involving benign nerve sheath tumors. The loss of SNF5/SMARCB1 disrupts the SWI/SNF complex's function triggering unregulated cell proliferation and cancer progression. Within these disorders the SWI/SNF complex itself which contains other subunits like BRG1 and BRM is also impacted highlighting SMARCB1's critical role in tumor suppression.

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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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Abcam Product Promise

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人SMARCB1 (SNF5) knockout HEK-293T cell裂解物

Human SMARCB1 (SNF5) knockout HEK-293T cell lysate

Western blot - Human SMARCB1 (SNF5) knockout HEK-293T cell lysate (AB257688)

Western blot - Human SMARCB1 (SNF5) knockout HEK-293T cell lysate (AB257688)

Sanger Sequencing - Human SMARCB1 (SNF5) knockout HEK-293T cell lysate (AB257688)

Sanger Sequencing - Human SMARCB1 (SNF5) knockout HEK-293T cell lysate (AB257688)