人SMAD7 (MADH7) knockout HeLa cell裂解物
Human SMAD7 (MADH7) knockout HeLa cell lysate
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SMAD7 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.
查看别名
CRCS3, FLJ16482, MAD (mothers against decapentaplegic Drosophila) homolog 7, MAD homolog 8, MAD mothers against decapentaplegic homolog 7, MADH 7, MADH 8, MADH6, Mad homolog 7, Mothers Against Decapentaplegic Drosophila Homolog of 6, Mothers Against Decapentaplegic Drosophila Homolog of 7, Mothers against DPP homolog 7, Mothers against DPP homolog 8, Mothers against decapentaplegic homolog 7, Mothers against decapentaplegic homolog 8, SMA- AND MAD-RELATED PROTEIN 7, SMAD, SMAD family member 7, SMAD, mothers against DPP homolog 7, SMAD, mothers against DPP homolog 7 (Drosophila), SMAD7_HUMAN, hSMAD 7
- Sanger seq
Unknown
Sanger Sequencing - Human SMAD7 (MADH7) knockout HeLa cell lysate (AB258686)
Homozygous : 1 bp insertion in exon4
产品详情
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
规格
性能和储存信息
基因名称
基因编辑类型
基因编辑方法
敲除验证
运输条件
推荐的短期储存条件
推荐的长期储存条件
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SMAD7 functions to regulate the TGF-beta signaling pathway by operating as a negative feedback mediator. It binds to TGF-beta receptors recruiting E3 ubiquitin ligases which promote receptor degradation. SMAD7 is not part of complex large-scale multi-protein assemblies but is essential in modulating the pathway's activity. Its balance controls important processes like cell proliferation differentiation and apoptosis across varied biological contexts.
Pathways
SMAD7 strongly influences the TGF-beta and BMP (bone morphogenetic protein) signaling pathways. SMAD7 blocks TGF-beta signaling by interfering with the activity of R-SMADs such as SMAD2 and SMAD3 and it modulates the BMP pathway by interacting with SMAD1 and SMAD5. Its regulatory roles are tightly integrated within these pathways highlighting its importance in cellular homeostasis and response to extracellular signals.
质量控制
STR 分析
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
细胞培养
生物安全等级
EU: 2 US: 2
贴壁/悬浮
Adherent
性别
Female
搭配使用产品
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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