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AB258184

人SCAMP1 knockout HeLa cell裂解物

Human SCAMP1 knockout HeLa cell lysate

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SCAMP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 25 bp deletion in exon2.

查看别名

SCAM1_HUMAN, SCAMP, SCAMP 37, Secretory carrier membrane protein, Secretory carrier membrane protein 1, Secretory carrier-associated membrane protein 1

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Western blot - Human SCAMP1 knockout HeLa cell lysate (AB258184)
  • WB

Lab

Western blot - Human SCAMP1 knockout HeLa cell lysate (AB258184)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : SCAMP1 knockout HeLa cell lysate 20 μg

Lanes 1 - 2 : Merged signal (red and green). Green - Anti-SCAMP1 antibody observed at 36 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa. Anti-SCAMP1 antibody was shown to react with SCAMP1 in wild-type HeLa cells in western blot. The bands observed in SCAMP1 knockout cell line ab265567 (SCAMP1 knockout cell lysate ab258184) below 36 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and SCAMP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with anti-SCAMP1 antibody and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

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Sanger Sequencing - Human SCAMP1 knockout HeLa cell lysate (AB258184)
  • Sanger seq

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Sanger Sequencing - Human SCAMP1 knockout HeLa cell lysate (AB258184)

Allele-1 : 25 bp deletion in exon2

Sanger Sequencing - Human SCAMP1 knockout HeLa cell lysate (AB258184)
  • Sanger seq

Unknown

Sanger Sequencing - Human SCAMP1 knockout HeLa cell lysate (AB258184)

Allele-2 : 1 bp insertion in exon2

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

敲除验证

Sanger Sequencing

突变描述

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 25 bp deletion in exon2.

疾病

Adenocarcinoma

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反应性数据

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称
SCAMP1
基因编辑类型
Knockout
基因编辑方法
CRISPR technology
敲除验证
Sanger Sequencing
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SCAMP1 short for Secretory Carrier Membrane Proteins 1 plays a mechanical role in the transport and recycling of membranes. It is a protein of approximately 38 kDa and typically localizes to the vesicular membranes within the cytoplasm. Expression of SCAMP1 can be observed in a variety of tissues including those of the nervous system and endocrine glands reflecting its involvement in intricate vesicular traffic processes.
Biological function summary

SCAMP1 is engaged in the regulation of endocytosis and exocytosis within cellular processes. It is not part of a conventional complex but interacts dynamically with other proteins involved in vesicle fusion and release. SCAMP1 associates with cytosolic domains and membrane proteins to orchestrate the trafficking of vesicles ensuring efficient cellular communication and molecular transport across cellular membranes.

Pathways

Proteins related to vesicular transport such as SNAREs interact functionally with SCAMP1. Within this context SCAMP1 is actively involved in the endocytic and exocytic pathways which are important for maintaining cellular homeostasis and neurotransmitter release. These pathways are vital for the release of neurotransmitters during synaptic signaling and hormone secretion in endocrine systems with SCAMP1 aiding in the precise regulation of these events.

Dysregulation of SCAMP1 associates with neurodegenerative conditions like Alzheimer's disease and metabolic disorders such as diabetes mellitus. SCAMP1's connection to SNARE proteins in these scenarios highlights its impactful role in synaptic and endocrine dysfunctions. In Alzheimer's disease altered vesicle recycling and transport influenced by SCAMP1 may contribute to impaired neuronal signaling pathways while in diabetes it affects insulin secretion dynamics important for glucose metabolism.
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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

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Complementary Products

Primary Antibodies

AB3430

Anti-SCAMP1 antibody

Western blot - Anti-SCAMP1 antibody (AB3430)

  • 0
  • 0
Cell Lines & Lysates

AB265567

Human SCAMP1 knockout HeLa cell line

Advanced Validation

Western blot - Human SCAMP1 knockout HeLa cell line (AB265567)

  • 0
  • 0

Abcam Product Promise

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详情请参阅我们的条款与条件。

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