人RAB1A knockout HEK-293T cell裂解物
Human RAB1A knockout HEK-293T cell lysate
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RAB1A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 8 bp deletion in exon 1.
查看别名
GTP binding protein RAB 1A, RAB 1, RAB1, member RAS oncogene family, RAB1A member RAS oncogene family, RAB1A_HUMAN, Ras-associated protein RAB1, Ras-related protein Rab-1A, YPT1, YPT1-related protein, mKIAA3012
- Sanger seq
Unknown
Sanger Sequencing - Human RAB1A knockout HEK-293T cell lysate (AB258615)
Allele-2 : 8 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human RAB1A knockout HEK-293T cell lysate (AB258615)
Allele-1 : 10 bp deletion in exon 1
产品详情
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
规格
性能和储存信息
基因名称
基因编辑类型
基因编辑方法
敲除验证
运输条件
推荐的短期储存条件
推荐的长期储存条件
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Rab1A protein influences intracellular transport processes and maintains cellular homeostasis. It forms part of multimeric protein complexes that facilitate its functions in the secretory pathway. The protein ensures the proper trafficking of cargo proteins from the endoplasmic reticulum to the Golgi apparatus playing a role in the early secretory pathway. This involvement is critical for maintaining the post-translational modification and processing of proteins.
Pathways
The mechanical function of Rab1A integrates into several cellular pathways including the secretory pathway and autophagy. Rab1A works with other proteins like Rab2 and Rab30 in the secretory pathway to coordinate the proper flow of proteins. Its coordination with proteins like LC3 during autophagy highlights its importance in cellular turnover and degradation processes linking Rab1A to essential biological functions and homeostasis.
质量控制
STR 分析
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
细胞培养
生物安全等级
EU: 2 US: 2
贴壁/悬浮
Adherent
性别
Female
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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