AB258135

人PPP2R5E knockout HeLa cell裂解物

Name: 人PPP2R5E knockout HeLa cell lysate (ab258135)| Abcam中文官网
Brand: Abcam Limited
SKU: AB258135
Price: 6206 CNY
Availability: InStock

Human PPP2R5E knockout HeLa cell lysate

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PPP2R5E KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.

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2A5E_HUMAN, Epsilon isoform of regulatory subunit B56 protein phosphatase 2A, PP2A B subunit B' epsilon, PP2A B subunit B' epsilon isoform, PP2A B subunit B56 epsilon, PP2A B subunit B56 epsilon isoform, PP2A B subunit PR61 epsilon, PP2A B subunit PR61 epsilon isoform, PP2A B subunit R5 epsilon, PP2A B subunit R5 epsilon isoform, PP2A B subunit isoform B''-epsilon, PP2A B subunit isoform B'-epsilon, PP2A B subunit isoform B56-epsilon, PP2A B subunit isoform PR61-epsilon, PP2A B subunit isoform R5-epsilon, PPP2R5E, Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform, Protein phosphatase 2 regulatory subunit B' epsilon, Protein phosphatase 2 regulatory subunit B' epsilon isoform, Regulatory subunit B of protein phosphatase 2 epsilon isoform, Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon, Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform

4 Images

Lab

Western blot - Human PPP2R5E knockout HeLa cell lysate (AB258135)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : PPP2R5E knockout HeLa cell lysate (20 μg)

Lane 3 : HEK-293 cell lysate (20 μg)

Lane 4 : Daudi cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab198290 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

ab198290 Anti-PPP2R5E antibody [EPR17146] was shown to specifically react with PPP2R5E in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265637 (knockout cell lysate ab258135) was used. Wild-type and PPP2R5E knockout samples were subjected to SDS-PAGE. ab198290 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PPP2R5E antibody [EPR17146] (<a href='/products/primary-antibodies/ppp2r5e-antibody-epr17146-ab198290'>ab198290</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PPP2R5E knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PPP2R5E knockout HeLa cell line (<a href='/products/cell-lines/human-ppp2r5e-knockout-hela-cell-line-ab265637'>ab265637</a>)

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Human PPP2R5E knockout HeLa cell lysate (AB258135)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : PPP2R5E knockout HeLa cell lysate (20 μg)

Lane 3 : HEK-293 cell lysate (20 μg)

Lane 4 : Daudi cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab198500 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

ab198500 Recombinant Anti-PPP2R5E antibody [EPR17147] - C-terminal was shown to specifically react with PPP2R5E in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265637 (knockout cell lysate ab258135) was used. Wild-type and PPP2R5E knockout samples were subjected to SDS-PAGE. ab198500 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PPP2R5E antibody [EPR17147] - C-terminal (<a href='/products/primary-antibodies/ppp2r5e-antibody-epr17147-c-terminal-ab198500'>ab198500</a>) at 1/5000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PPP2R5E knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PPP2R5E knockout HeLa cell line (<a href='/products/cell-lines/human-ppp2r5e-knockout-hela-cell-line-ab265637'>ab265637</a>)

Lane 3:

HEK-293T cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Sanger Sequencing - Human PPP2R5E knockout HeLa cell lysate (AB258135)

Sanger seq

Unknown

Sanger Sequencing - Human PPP2R5E knockout HeLa cell lysate (AB258135)

Allele-2 : Insertion of the selection cassette in exon2

Sanger seq

Unknown

Sanger Sequencing - Human PPP2R5E knockout HeLa cell lysate (AB258135)

Allele-1 : 1 bp deletion in exon2

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.

疾病

Adenocarcinoma

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258135-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human PPP2R5E knockout HeLa cell lysate", "number":"AB258135-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258135-CMP02", "productcode":"" } ] } } }

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性能和储存信息

基因名称

PPP2R5E

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Sanger Sequencing, Western blot

运输条件

Ambient - Can Ship with Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein PPP2R5E also known as B56 epsilon is a regulatory subunit of protein phosphatase 2A (PP2A). It has an approximate mass of 72 kDa. PPP2R5E expresses across various tissues including the brain heart and skeletal muscle. The subunit associates with the core PP2A enzyme influencing substrate specificity and activity of PP2A holoenzyme.

Biological function summary

PPP2R5E plays a role in regulating cell growth and division. It is a part of the PP2A holoenzyme complex acting as a critical modulatory component. By associating with the PP2A core enzyme PPP2R5E contributes to the dephosphorylation process of many substrates. These substrates are vital for maintaining cellular functions such as signal transduction and cell cycle progression.

Pathways

PPP2R5E takes part in cell signaling and apoptotic pathways. It has substantial involvement in the MAPK signaling pathway where it modulates the activity of related kinases. PPP2R5E interacts with proteins like RAF1 influencing the pathway's function. Additionally it also participates in the Wnt signaling pathway where its regulatory role impacts cellular differentiation and proliferation.

PPP2R5E shows links to cancer and neurodegenerative diseases. Alterations in its expression or function may lead to oncogenesis due to its role in controlling cell proliferation signals. The dysregulation of PP2A activity potentially through PPP2R5E has associations with Alzheimer’s disease. In cancer PPP2R5E interacts with proteins such as c-Myc which contributes to tumorigenesis and progression.

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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

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For licensing inquiries, please contact partnerships@abcam.com

人PPP2R5E knockout HeLa cell裂解物

Human PPP2R5E knockout HeLa cell lysate

Western blot - Human PPP2R5E knockout HeLa cell lysate (AB258135)

Western blot - Human PPP2R5E knockout HeLa cell lysate (AB258135)

Sanger Sequencing - Human PPP2R5E knockout HeLa cell lysate (AB258135)

Sanger Sequencing - Human PPP2R5E knockout HeLa cell lysate (AB258135)