人PPM1A knockout HeLa cell裂解物
Human PPM1A knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
PPM1A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon3 and Insertion of the selection cassette in exon3.
查看别名
EC 3.1.3.16, FLJ42306, IA, MGC9201, Mpp alpha, PP2C-alpha, PP2CA, PPM1A_HUMAN, PPPM1A, Protein phosphatase 1A, Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform, Protein phosphatase 1A magnesium dependent alpha, Protein phosphatase 2C alpha, Protein phosphatase 2C alpha isoform, Protein phosphatase 2C isoform alpha, Protein phosphatase IA, Protein phosphatase, Mg2+/Mn2+ dependent, 1A
- WB
Lab
Western blot - Human PPM1A knockout HeLa cell lysate (AB259055)
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20 ug)
Lane 2 : PPM1A knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20 ug)
Lane 3 : HAP1 whole cell lyate (20 ug)
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (20 ug)
ab173527 was shown to specifically react with GNAI3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265348 (knockout cell lysate ab259055) was used. Wild-type and GNAI3 knockout samples were subjected to SDS-PAGE. ab173527 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4oC at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PPM1A antibody [p6c7] (<a href='/products/primary-antibodies/ppm1a-antibody-p6c7-ab14824'>ab14824</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2:
PPM1A knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2:
Western blot - Human PPM1A knockout HeLa cell line (<a href='/products/cell-lines/human-ppm1a-knockout-hela-cell-line-ab265348'>ab265348</a>)
Lane 3:
HAP1 whole cell lyate at 20 µg
Lane 4:
Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution
Predicted band size: 41 kDa,42 kDa
Observed band size: 42 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PPM1A knockout HeLa cell lysate (AB259055)
Allele-1 : 4 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human PPM1A knockout HeLa cell lysate (AB259055)
Allele-2 : Insertion of the selection cassette in exon3
反应性数据
产品详情
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
规格
性能和储存信息
基因名称
基因编辑类型
基因编辑方法
敲除验证
运输条件
推荐的短期储存条件
推荐的长期储存条件
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PPM1A regulates many cellular mechanisms by dephosphorylating targets and acting as a negative regulator of several signaling pathways. It modulates stress responses cell cycle progression and immune responses. PPM1A does not typically form complexes with other proteins but interacts closely with specific signaling molecules to control their activity. This regulation influences cellular homeostasis and growth in response to external and internal signals.
Pathways
PPM1A influences the TGF-beta signaling pathway where it interacts with and deactivates SMAD proteins by dephosphorylation. It also participates in the p38 MAPK pathway. These interactions highlight its role in controlling transduction signals initiated by growth factors and stress. By regulating these pathways PPM1A maintains a balance in cellular responses to growth and environmental stresses.
质量控制
STR 分析
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
细胞培养
生物安全等级
EU: 2 US: 2
贴壁/悬浮
Adherent
性别
Female
搭配使用产品
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1A antibody [p6c7] (AB14824)](https://content.abcam.com/products/images/ppm1a-antibody-p6c7-ab14824--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73582.jpg)
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com