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AB259040

人PIK3C2A (PI 3 Kinase Class 2A/Cpk) knockout HeLa cell裂解物

Human PIK3C2A (PI 3 Kinase Class 2A/Cpk) knockout HeLa cell lysate

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PIK3C2A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 43 bp insertion in exon 1.

查看别名

C2 containing phosphatidylinositol kinase, C2 domain containing alpha polypeptide, CPK, Class 2 alpha polypeptide, Cpk m, DKFZp686L193, MGC142218, OTTHUMP00000231763, P3C2A_HUMAN, PI3-K-C2(ALPHA), PI3-K-C2A, PI3K-C2-alpha, PIK3C 2A, Phosphatidylinositol 3 kinase class 2 alpha, Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha, Phosphoinositide 3 kinase class 2 alpha, Phosphoinositide 3 kinase, class 2, alpha polypeptide, Phosphoinositide 3-Kinase-C2-alpha, PtdIns-3-kinase C2 subunit alpha

1 Images
Sanger Sequencing - Human PIK3C2A (PI 3 Kinase Class 2A/Cpk) knockout HeLa cell lysate (AB259040)
  • Sanger seq

Unknown

Sanger Sequencing - Human PIK3C2A (PI 3 Kinase Class 2A/Cpk) knockout HeLa cell lysate (AB259040)

Homozygous : 43 bp insertion in exon 1

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

敲除验证

Sanger Sequencing

突变描述

Knockout achieved by using CRISPR/Cas9, Homozygous: 43 bp insertion in exon 1.

疾病

Adenocarcinoma

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称
PIK3C2A
基因编辑类型
Knockout
基因编辑方法
CRISPR technology
敲除验证
Sanger Sequencing
合子性
Homozygous
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PI 3 Kinase Class 2A also known as Cpk is a significant enzyme involved in the regulation of various cellular processes. The target weighing approximately 170 kDa acts as a lipid kinase that phosphorylates phosphatidylinositol converting PI to PI3P. Cpk expresses mainly in tissues like the liver muscle and heart where it contributes to cellular homeostasis and metabolic regulation.
Biological function summary

This enzyme family modulates intracellular signaling cascades influencing processes such as cell growth survival and metabolism. Cpk forms part of a larger complex that interacts with adaptor proteins facilitating its recruitment to cellular membranes. This interaction is important for its signaling activity and function in maintaining cellular equilibrium.

Pathways

PI 3 Kinase Class 2A plays an integral role in the insulin signaling pathway and the TOR signaling pathway. In the insulin pathway it collaborates with proteins like Akt to modulate glucose uptake and storage in cells. Similarly in the TOR pathway Cpk contributes to nutrient sensing and cell growth regulation interconnected with proteins such as mTOR affecting protein synthesis and autophagy.

Dysregulation of PI 3 Kinase Class 2A links to cancer and type 2 diabetes. Alterations in its activity may lead to abnormal cell proliferation and survival characteristics of cancerous transformations associated with proteins like PTEN. Meanwhile its role in glucose metabolism ties it to insulin resistance and diabetes pathogenesis where interactions with insulin receptor substrates become important for disease progression.
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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

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搭配使用产品

Cell Lines & Lysates

AB264943

Human PIK3C2A (PI 3 Kinase Class 2A/Cpk) knockout HeLa cell line

Sanger Sequencing - Human PIK3C2A (PI 3 Kinase Class 2A/Cpk) knockout HeLa cell line (AB264943)

  • 0
  • 0
Primary Antibodies

AB40776

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (AB40776)

  • 0
  • 1
Primary Antibodies

AB307099

Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] - BSA and Azide Free

Recombinant

Western blot - Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] - BSA and Azide Free (AB307099)

  • 0
  • 0
Primary Antibodies

AB278545

Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

20ul selling size|Recombinant

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

  • 5
  • 1

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