人PACSIN2 knockout HeLa cell裂解物
Human PACSIN2 knockout HeLa cell lysate
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PACSIN2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 8 bp deletion in exon 3.
查看别名
PACSIN2, Syndapin-2, Protein kinase C and casein kinase substrate in neurons protein 2, SdpII, Syndapin-II
- Sanger seq
Unknown
Sanger Sequencing - Human PACSIN2 knockout HeLa cell lysate (AB258558)
Allele-2 : 1 bp insertion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human PACSIN2 knockout HeLa cell lysate (AB258558)
Allele-1 : 8 bp deletion in exon 3
搭配使用产品
Primary Antibodies
AB237385
Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63]
RabMAb|Recombinant
![Flow Cytometry - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63] (AB237385)](https://content.abcam.com/products/images/alexa-fluor-647-epcam-antibody-epr20533-63-ab237385--flow-cytometry-img37274.jpg)
- 0
- 0
产品详情
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
规格
性能和储存信息
基因名称
基因编辑类型
基因编辑方法
敲除验证
运输条件
推荐的短期储存条件
推荐的长期储存条件
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PACSIN2 regulates cytoskeletal dynamics and membrane trafficking. It acts as a part of a protein complex that includes dynamin which is essential for the fission of vesicular membranes from the plasma membrane during endocytosis. Through its involvement in these processes PACSIN2 assists in maintaining cellular morphology and transport mechanisms within cells. Its interaction with dynamin and actin indicates its role in modulating cellular infrastructure during response to environmental changes or cellular signals.
Pathways
PACSIN2 participates in the endocytic pathway and the regulation of cytoskeletal dynamics. It interacts with proteins like dynamin important in vesicle scission during endocytosis. Moreover PACSIN2 influences pathways related to actin dynamics and cellular morphology changes interacting with actin to coordinate cytoskeletal rearrangements. This involvement highlights its function in cellular adaptation to different stimuli.
质量控制
STR 分析
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
细胞培养
生物安全等级
EU: 2 US: 2
贴壁/悬浮
Adherent
性别
Female
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com