人OTUD4 (HIN-1) knockout HeLa cell裂解物
Human OTUD4 (HIN-1) knockout HeLa cell lysate
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OTUD4 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and Insertion of the selection cassette in exon1.
查看别名
DKFZp434I0721, DUBA6, HIN1, HIV 1 INTEGRATION 1, HIV-1-induced protein HIN-1, HSHIN, HSHIN1, KIAA1046, OTU domain containing 4, OTU domain containing 4 protein, OTU domain-containing protein 4, OTUD4_HUMAN
- Sanger seq
Unknown
Sanger Sequencing - Human OTUD4 (HIN-1) knockout HeLa cell lysate (AB263289)
Allele-1 : 2 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human OTUD4 (HIN-1) knockout HeLa cell lysate (AB263289)
Allele-2 : Insertion of the selection cassette in exon1
产品详情
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
规格
性能和储存信息
基因名称
基因编辑类型
基因编辑方法
敲除验证
运输条件
推荐的短期储存条件
推荐的长期储存条件
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The OTUD4 protein significantly influences cellular homeostasis and stress response. It participates in the protein quality control system as part of a complex that regulates the ubiquitin-proteasome system. This protein ensures the proper folding and degradation of misfolded proteins impacting cellular health and function. OTUD4 forms interactions with other proteins highlighting its role in modulating cellular stress responses and apoptosis.
Pathways
OTUD4 has connections with both the ubiquitin-proteasome and DNA damage response pathways. It participates in the modulation of these pathways ensuring that damaged or misfolded proteins do not accumulate. OTUD4 directly interacts with proteins such as ubiquitin-specific proteases and E3 ligases influencing apoptosis and stress response. These interactions reveal its involvement in cellular decisions related to repair survival or apoptosis.
质量控制
STR 分析
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
细胞培养
生物安全等级
EU: 2 US: 2
贴壁/悬浮
Adherent
性别
Female
搭配使用产品
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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