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AB259021

人OSTC knockout HEK-293T cell裂解物

Human OSTC knockout HEK-293T cell lysate

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OSTC KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1.

查看别名

DC2, HDCMD45P, HSPC307, Hydrophobic protein HSF-28, OSTC_HUMAN, Oligosaccharyltransferase complex subunit, Oligosaccharyltransferase complex subunit OSTC

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Sanger Sequencing - Human OSTC knockout HEK-293T cell lysate (AB259021)
  • Sanger seq

Unknown

Sanger Sequencing - Human OSTC knockout HEK-293T cell lysate (AB259021)

Homozygous : 7 bp deletion in exon 1

关键信息

细胞类型

HEK-293T

种属

Human

组织

Kidney

敲除验证

Sanger Sequencing

突变描述

Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1.

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称
OSTC
基因编辑类型
Knockout
基因编辑方法
CRISPR technology
敲除验证
Sanger Sequencing
合子性
Homozygous
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

OSTC also known as oligosaccharyltransferase complex subunit is a component in the N-linked glycosylation pathway. It has a molecular mass of approximately 16 kDa. OSTC is located primarily in the endoplasmic reticulum and displays a ubiquitous expression indicating its presence in various cell types and tissues. This protein is an important player in the assembly and function of the oligosaccharyltransferase complex which catalyzes the transfer of oligosaccharides onto nascent peptides a process important for proper protein folding and stability.
Biological function summary

The oligosaccharyltransferase complex including OSTC plays a significant role in protein modification. The complex facilitates the attachment of glycans to asparagine residues on target proteins an essential post-translational modification process. Being a part of this multi-protein complex OSTC contributes actively to maintaining cellular homeostasis by influencing protein quality control and secretion.

Pathways

OSTC's involvement is embedded in the N-glycosylation pathway which is important for the proper folding and function of numerous glycoproteins. This pathway intersects with the protein processing pathway in the endoplasmic reticulum where OSTC collaborates with other protein components like OST48 and ribophorin ensuring efficient protein maturation. These interactions highlight OSTC's role in maintaining cellular function through its integration into key biochemical pathways.

Several studies link OSTC to congenital disorders of glycosylation which arise from dysfunctional glycosylation processes. Abnormal OSTC expression or function may result in disrupted protein folding and stability contributing to neurodevelopmental disorders and immunodeficiencies. Furthermore OSTC's interaction with proteins involved in the glycosylation pathway can accentuate phenotypic manifestations in ER stress-related diseases highlighting the importance of this target in clinical research and therapy development.
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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

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