AB257245

人NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell裂解物

Name: 人NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (ab257245)| Abcam中文官网
Brand: Abcam Limited
SKU: AB257245
Price: 5956 CNY
Availability: InStock

Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate

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NFKB2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon8.

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CVID10, DNA-binding factor KBF2, H2TF1, LYT 10, Lymphocyte translocation chromosome 10 protein, NFKB p52/p100 subunit, Nuclear factor Kappa B subunit 2, Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100), Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10, Transcription factor NFKB2

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Lab

Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (AB257245)

Lane 1 : Wild-type HCT116 cell lysate (20μg)
Lane 2 : NFKB2 knockout HCT116 cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab109440 observed at 120 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab109440 Anti-NFkB p100/NFKB2 antibody [EPR4686] was shown to specifically react with NFkB p100/NFKB2 in wild-type HCT116 cells in western blot. The band observed in the knockout cell line ab266883 (knockout cell lysate ab257245) lane below 97kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and NFkB p100/NFKB2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109440 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686] (<a href='/products/primary-antibodies/nfkb-p100-nfkb2-antibody-epr4686-ab109440'>ab109440</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT116 cell lysate at 20 µg

Lane 2:

NFKB2 knockout HCT116 cell lysate at 20 µg

Predicted band size: 97 kDa

Observed band size: 120 kDa

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Sanger Sequencing - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (AB257245)

Sanger seq

Unknown

Sanger Sequencing - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (AB257245)

Homozygous : 5 bp deletion in exon8

Lab

Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (AB257245)

Lane 1 : Wild-type HCT116 cell lysate (20μg)
Lane 2 : NFKB2 knockout HCT117 cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab175192 observed at 120 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab175192 Anti-NFkB p100/NFKB2 antibody [EPR4686-66] was shown to specifically react with NFkB p100/NFKB2 in wild-type HCT116 cells in western blot. The band observed in the knockout cell line ab266883 (knockout cell lysate ab257245) lane below 97kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and NFkB p100/NFKB2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab175192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NFkB p100/NFKB2 antibody [EPR4686-66] (<a href='/products/primary-antibodies/nfkb-p100-nfkb2-antibody-epr4686-66-ab175192'>ab175192</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT116 cell lysate at 20 µg

Lane 2:

Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (ab257245)

Predicted band size: 97 kDa

Observed band size: 120 kDa

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关键信息

细胞类型

HCT116

种属

Human

组织

Colon

敲除验证

Sanger Sequencing

突变描述

Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon8.

疾病

Carcinoma

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.</p>" } } }

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产品详情

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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规格

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257245-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human NFKB2 knockout HCT116 cell lysate", "number":"AB257245-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HCT116 cell lysate", "number":"AB257245-CMP02", "productcode":"" } ] } } }

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性能和储存信息

基因名称

NFKB2

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Sanger Sequencing

合子性

Homozygous

运输条件

Ambient - Can Ship with Ice

质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 1 US: 1

贴壁/悬浮

Adherent

性别

Male

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

人NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell裂解物

Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate

Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (AB257245)

Sanger Sequencing - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (AB257245)

Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell lysate (AB257245)