人NFATC3 (NFAT4) knockout HEK-293T cell裂解物
Human NFATC3 (NFAT4) knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
NFATC3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3.
查看别名
NFATc3, NF-ATc3, T-cell transcription factor NFAT4, NFAT4, NFATC3, NF-AT4c, NF-AT4, NFATx
- WB
Lab
Western blot - Human NFATC3 (NFAT4) knockout HEK-293T cell lysate (AB258073)
Lane 1 : Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate, 40 ug
Lane 2 : NFATC3 (NFAT4) knockout HEK293T (human embryonic kidney epithelial cell) whole cell lysate, 40 ug
Lanes 1- 2 : Merged signal (red and green). Green - ab283690 observed at 140 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab283690 was shown to react with NFATC3 (NFAT4) in wild-type HEK293T cells in western blot. Loss of signal was observed when NFATC3 (NFAT4) knockout cell line ab267300 (knockout cell lysate ab258073) was used. Wild-type HEK293T and NFATC3 (NFAT4) knockout HEK293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab283690 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4°C overnight at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Lysates were made freshly and used in WB immediately to minimize protein degradation.
Blocking and diluting buffer and concentration : 3% NFDM/TBST
All lanes:
Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (<a href='/products/primary-antibodies/nfat4-nf-atc3-antibody-epr24994-68-ab283690'>ab283690</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate at 40 µg
Lane 2:
NFATC3 (NFAT4) knockout HEK293T (human embryonic kidney epithelial cell) whole cell lysate at 40 µg
Lane 2:
Western blot - Human NFATC3 (NFAT4) knockout HEK-293T cell line (<a href='/products/cell-lines/human-nfatc3-nfat4-knockout-hek-293t-cell-line-ab267300'>ab267300</a>)
Secondary
All lanes:
Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution
Predicted band size: 116 kDa
Observed band size: 140 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human NFATC3 (NFAT4) knockout HEK-293T cell lysate (AB258073)
Homozygous : 1 bp insertion in exon3
搭配使用产品
Primary Antibodies
AB283690
Anti-NFAT4/NF-ATc3 antibody [EPR24994-68]
20ul selling size|RabMAb|Recombinant|KO Validated
![Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (AB283690)](https://content.abcam.com/products/images/nfat4-nf-atc3-antibody-epr24994-68-ab283690--western-blot-img172334.jpg)
- 0
- 0
反应性数据
产品详情
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
规格
性能和储存信息
基因名称
基因编辑类型
基因编辑方法
敲除验证
运输条件
推荐的短期储存条件
推荐的长期储存条件
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NFAT4/NF-ATc3 acts as a regulator of gene expression by binding to DNA in the nucleus. It forms part of the NFAT complex working with other members of this family to regulate genes involved in cell differentiation and development. In immune cells this protein plays a role in mediating responses to external signals influencing cytokine production and aiding the regulation of immune responses. Other tissues including neurons and cardiac cells also rely on NFAT4 for specific signaling functions related to cell growth and survival.
Pathways
NFAT4/NF-ATc3 is involved in key signaling mechanisms notably the calcium signaling pathway. This pathway works closely with other proteins such as calcineurin which dephosphorylates NFAT proteins leading to their activation and nuclear translocation. Additionally interactions within the Ras/MAPK pathway highlight NFAT4's role in cell proliferation and differentiation processes interconnecting with various signaling proteins that drive these pathways.
质量控制
STR 分析
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
细胞培养
生物安全等级
EU: 2 US: 2
贴壁/悬浮
Adherent
性别
Female
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com