AB258059

人NCK1 (Nck) knockout HeLa cell裂解物

Name: 人NCK1 (Nck) knockout HeLa cell lysate (ab258059)| Abcam中文官网
Brand: Abcam Limited
SKU: AB258059
Price: 6145 CNY
Availability: InStock

Human NCK1 (Nck) knockout HeLa cell lysate

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NCK1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.

查看别名

Cytoplasmic protein NCK1, MGC12668, Melanoma Nck protein, NCK, NCK adaptor protein 1, NCK alpha, NCK tyrosine kinase, NCK1_HUMAN, Non catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK-alpha

2 Images

Lab

Western blot - Human NCK1 (Nck) knockout HeLa cell lysate (AB258059)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : NCK1 knockout HeLa cell lysate (20 ug)
Lane 3 : NIH/3T3 cell lysate (20 ug)

ab32120 was shown to specifically react with Nck in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265323 (knockout cell lysate ab258059) was used. Wild-type and Nck knockout samples were subjected to SDS-PAGE. ab32120 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Nck antibody [Y531] (<a href='/products/primary-antibodies/nck-antibody-y531-ab32120'>ab32120</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

NCK1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human NCK1 (Nck) knockout HeLa cell line (<a href='/products/cell-lines/human-nck1-nck-knockout-hela-cell-line-ab265323'>ab265323</a>)

Lane 3:

NIH/3T3 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 50 kDa

false

Sanger Sequencing - Human NCK1 (Nck) knockout HeLa cell lysate (AB258059)

Sanger seq

Unknown

Sanger Sequencing - Human NCK1 (Nck) knockout HeLa cell lysate (AB258059)

Homozygous : 1 bp insertion in exon2

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.

疾病

Adenocarcinoma

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称

NCK1

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Sanger Sequencing, Western blot

运输条件

Ambient - Can Ship with Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Nck protein also referred to as Nck1 n c k or Nck is an adaptor protein integral in cell signaling pathways. It consists of a molecular mass around 45 kDa and exists mainly within the cytoplasm. The Nck proteins including Nck1 and its closely related form Nck2 are particularly prominent in cells of the immune and nervous systems. Functionally they facilitate interactions between receptor tyrosine kinases and downstream signaling molecules translating extracellular stimuli into cellular responses.

Biological function summary

The Nck proteins play significant roles in actin cytoskeleton reorganization influencing cell shape movement and adhesion. These proteins often participate as part of larger signaling complexes where they serve as scaffolds bringing together key molecules. Nck proteins directly interact with partners containing SH2 and SH3 domains which are important for various cellular dynamic processes. Their role in cytoskeletal dynamics aligns them with many cellular mechanisms including endocytosis and cell motility.

Pathways

Nck proteins significantly influence the regulation of the actin cytoskeleton pathway and the Rho GTPases pathway. Through these pathways they interact with proteins such as WASP and Rac regulating actin polymerization and cell migration. Nck’s ability to form complex signaling networks positions it as a central actor in transducing signals that affect cell shape and movement. It acts collaboratively with multiple signaling pathways to maintain cellular plasticity and response to extracellular signals.

Nck proteins have associations with cancer and immunological conditions. Alterations in their signaling functions may contribute to tumor progression or metastasis given their role in cell movement and adhesion. In immune disorders abnormal Nck signaling could disrupt normal immune cell functions potentially leading to autoimmune conditions. For example Nck proteins may interact with oncogenic proteins like BCR-ABL in chronic myeloid leukemia highlighting their relevance in disease contexts.

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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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Abcam Product Promise

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人NCK1 (Nck) knockout HeLa cell裂解物

Human NCK1 (Nck) knockout HeLa cell lysate

Western blot - Human NCK1 (Nck) knockout HeLa cell lysate (AB258059)

Sanger Sequencing - Human NCK1 (Nck) knockout HeLa cell lysate (AB258059)