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AB258058

人NASP knockout HEK-293T cell裂解物

Human NASP knockout HEK-293T cell lysate

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NASP KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 27 bp deletion in exon 1.

查看别名

NASP, Nuclear autoantigenic sperm protein

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Sanger Sequencing - Human NASP knockout HEK-293T cell lysate (AB258058)
  • Sanger seq

Unknown

Sanger Sequencing - Human NASP knockout HEK-293T cell lysate (AB258058)

Homozygous : 27 bp deletion in exon 1

关键信息

细胞类型

HEK-293T

种属

Human

组织

Kidney

敲除验证

Sanger Sequencing

突变描述

Knockout achieved by using CRISPR/Cas9, Homozygous: 27 bp deletion in exon 1.

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搭配使用产品

Primary Antibodies

AB181169

Anti-NASP antibody [EPR12827]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-NASP antibody [EPR12827] (AB181169)

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Cell Lines & Lysates

AB266303

Human NASP knockout HEK-293T cell line

Sanger Sequencing - Human NASP knockout HEK-293T cell line (AB266303)

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称
NASP
基因编辑类型
Knockout
基因编辑方法
CRISPR technology
敲除验证
Sanger Sequencing
合子性
Homozygous
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NASP also known as Nuclear Autoantigenic Sperm Protein functions as a histone chaperone. With a molecular mass of approximately 75 kDa NASP assists in the proper assembly and disassembly of nucleosome structures by binding to histones H1 and H2A-H2B. This protein mainly expresses in rapidly dividing tissues especially in the testes but also presents in the thymus and bone marrow. NASP maintains the supply of free histones aiding in DNA replication and transcription processes.
Biological function summary

NASP plays an essential role in chromatin organization and dynamics during the cell cycle. As part of the histone transport complex it stabilizes histones and prevents their aggregation. NASP facilitates proper chromatin assembly necessary for DNA packaging and regulation of gene expression. This activity ensures the accurate transmission of genetic information during cell division impacting processes essential for cell proliferation and function.

Pathways

NASP operates within the chromatin assembly and nucleosome remodeling pathways. It coordinates with other chaperones like NAP1 in chromatin assembly factor-1 (CAF-1) complex. These pathways are integral to DNA replication and repair directly linking NASP to cellular processes critical for maintaining genome integrity. The interaction with importin beta further indicates NASP's role in nuclear import pathways emphasizing its involvement in both nuclear organization and cellular regulation.

NASP associates with cancer and infertility. The aberrant expression of NASP is observed in various tumors implying its contribution to tumorigenesis and proliferation. Its association with other histone chaperones such as ASF1 highlights its potential role in oncogenic pathways. Furthermore alterations in NASP expression affect sperm development linking it to male infertility and emphasizing its necessity in reproductive biology.
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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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