AB257194

人MTAP knockout HeLa cell裂解物

Name: 人MTAP knockout HeLa cell lysate (ab257194)| Abcam中文官网
Brand: Abcam Limited
SKU: AB257194
Price: 6206 CNY
Availability: InStock

Human MTAP knockout HeLa cell lysate

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MTAP KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.

查看别名

5' methylthioadenosine phosphorylase, 5''-methylthioadenosine phosphorylase, BDMF, DMSFH, DMSMFH, Epididymis luminal protein 249, HEL 249, LGMBF, MSAP, MTA phosphorylase, MTAP_HUMAN, MTAPase, MeSAdo phosphorylase, Methylthioadenosine phosphorylase, S methyl 5 thioadenosine phosphorylase, S methyl 5' thioadenosine phosphorylase, S-methyl-5''-thioadenosine phosphorylase, c86fus

5 Images

Unknown

Western blot - Human MTAP knockout HeLa cell lysate (AB257194)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : MTAP knockout HeLa cell lysate (20 μg)

Lane 3 : HT-29 cell lysate (20 μg)

Lane 4 : A549 cell lysate (20 μg)

anes 1- 4 : Merged signal (red and green). Green - ab126770 observed at 32 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab126770 Anti-MTAP antibody [EPR6893] was shown to specifically react with MTAP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265272 (knockout cell lysate ab257194) was used. Wild-type and MTAP knockout samples were subjected to SDS-PAGE. ab126770 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MTAP antibody [EPR22570-76] (<a href='/products/primary-antibodies/mtap-antibody-epr22570-76-ab254265'>ab254265</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MTAP knockout HeLa cell lysate at 20 µg

Lane 3:

HT-29 cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Predicted band size: 31 kDa

Observed band size: 32 kDa

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Unknown

Western blot - Human MTAP knockout HeLa cell lysate (AB257194)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : MTAP knockout HeLa cell lysate (20 μg)

Lane 3 : HT-29 cell lysate (20 μg)

Lane 4 : A549 cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab254265 observed at 32 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab254265 Anti-MTAP antibody [EPR22570-76] was shown to specifically react with MTAP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265272 (knockout cell lysate ab257194) was used. Wild-type and MTAP knockout samples were subjected to SDS-PAGE. ab254265 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MTAP antibody [EPR22570-76] (<a href='/products/primary-antibodies/mtap-antibody-epr22570-76-ab254265'>ab254265</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MTAP knockout HeLa cell lysate at 20 µg

Lane 3:

HT-29 cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Predicted band size: 31 kDa

Observed band size: 32 kDa

false

Sanger Sequencing - Human MTAP knockout HeLa cell lysate (AB257194)

Sanger seq

Unknown

Sanger Sequencing - Human MTAP knockout HeLa cell lysate (AB257194)

Allele-2 : Insertion of the selection cassette in exon1

Sanger seq

Unknown

Sanger Sequencing - Human MTAP knockout HeLa cell lysate (AB257194)

Allele-1 : 1 bp insertion in exon1

Sanger seq

Unknown

Sanger Sequencing - Human MTAP knockout HeLa cell lysate (AB257194)

Allele-3 : Insertion of the selection cassette in exon1

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.

疾病

Adenocarcinoma

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反应性数据

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称

MTAP

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Sanger Sequencing, Western blot

运输条件

Ambient - Can Ship with Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MTAP also known as methylthioadenosine phosphorylase is an essential enzyme that breaks down 5'-methylthioadenosine (MTA) a byproduct of polyamine synthesis. MTAP has a molecular weight of about 31 kDa. The enzyme converts MTA into adenine and 5-methylthioribose-1-phosphate which re-enter the methionine and adenine salvage pathways. MTAP is widely expressed in most tissues but its activity is especially high in the liver and kidney. Alternative names for MTAP include 2G4 and MTAP-A.

Biological function summary

Methylthioadenosine phosphorylase plays a significant role in the salvage pathways for methionine and adenine critical for cellular growth and proliferation. MTAP operates as a part of a complex metabolic network involved in polyamine metabolism. In addition to its metabolic functions MTAP contributes to the regulation of the immune response and cell cycle. MTAP immunohistochemistry is often used to study its expression patterns in various tissues.

Pathways

Methylthioadenosine phosphorylase participates importantly in the polyamine biosynthesis and methionine salvage pathways. These pathways are integral for maintaining cellular homeostasis and nucleotide pools. MTAP works closely with proteins such as methionine adenosyltransferase (MAT) and adenosylmethionine decarboxylase (AMD). In concert they facilitate the regeneration of methionine highlighting MTAP's role in cellular adaptation to metabolic demands.

Methylthioadenosine phosphorylase deficiency or deletion is linked to certain cancers such as gliomas and lymphomas. Loss of MTAP function is often associated with co-deletion of the tumor suppressor protein p16INK4a observed in various malignancies. This deletion can lead to an accumulation of MTA creating a toxic environment that promotes cancer cell proliferation. MTAP and its interaction with proteins like p16INK4a highlight its relevance as a potential target for therapeutic intervention in cancer treatment programs.

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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

人MTAP knockout HeLa cell裂解物

Human MTAP knockout HeLa cell lysate

Western blot - Human MTAP knockout HeLa cell lysate (AB257194)

Western blot - Human MTAP knockout HeLa cell lysate (AB257194)

Sanger Sequencing - Human MTAP knockout HeLa cell lysate (AB257194)

Sanger Sequencing - Human MTAP knockout HeLa cell lysate (AB257194)

Sanger Sequencing - Human MTAP knockout HeLa cell lysate (AB257194)