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AB257435

人FCGRT (FCRN) knockout HEK-293T cell裂解物

Human FCGRT (FCRN) knockout HEK-293T cell lysate

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FCGRT KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 3.

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Neonatal Fc receptor, FCGRT, FCRN, IgG receptor FcRn large subunit p51, IgG Fc fragment receptor transporter alpha chain, FcRn

2 Images
Western blot - Human FCGRT (FCRN) knockout HEK-293T cell lysate (AB257435)
  • WB

Lab

Western blot - Human FCGRT (FCRN) knockout HEK-293T cell lysate (AB257435)

Lanes 1-4 : Merged signal (red and green). Green - ab228975 observed at 40 kDa. Red - loading control ab8245 observed at 38 kDa.

ab228975 Anti-FCGRT/FCRN antibody [EPR22627-25] was shown to specifically react with FCGRT/FCRN in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab265075 (knockout cell lysate ab257435) was used. Wild-type and FCGRT/FCRN knockout samples were subjected to SDS-PAGE. ab228975 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4° at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FCGRT/FCRN antibody [EPR22627-25] (<a href='/products/primary-antibodies/fcgrt-fcrn-antibody-epr22627-25-ab228975'>ab228975</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (non-ATCC) cell lysate at 20 µg

Lane 2:

Western blot - Human FCGRT (FCRN) knockout HEK-293T cell lysate (ab257435)

Lane 2:

Human FCGRT (FCRN) knockout HEK-293T (non-ATCC) cell lysate (ab257435)

Lane 3:

HEK293 (ATCC) cell lysate at 20 µg

Lane 4:

LNCaP cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

Sanger Sequencing - Human FCGRT (FCRN) knockout HEK-293T cell lysate (AB257435)
  • Sanger seq

Unknown

Sanger Sequencing - Human FCGRT (FCRN) knockout HEK-293T cell lysate (AB257435)

Homozygous : 14 bp deletion in exon 3

关键信息

细胞类型

HEK-293T

种属

Human

组织

Kidney

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 3.

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搭配使用产品

Primary Antibodies

AB228975

Anti-FCGRT/FCRN antibody [EPR22627-25]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-FCGRT/FCRN antibody [EPR22627-25] (AB228975)

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Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

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Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

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Cell Lines & Lysates

AB265075

Human FCGRT (FCRN) knockout HEK-293T cell line

Advanced Validation

Western blot - Human FCGRT (FCRN) knockout HEK-293T cell line (AB265075)

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Proteins & Peptides

AB288799

Recombinant Human VCAM1 protein (Active)

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Functional Studies - Recombinant Human VCAM1 protein (Active) (AB288799)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称
FCGRT
基因编辑类型
Knockout
基因编辑方法
CRISPR technology
敲除验证
Sanger Sequencing, Western blot
合子性
Homozygous
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein FCGRT also known as FCRN or FcRn has a significant role in the immune system by binding to the Fc region of immunoglobulin G (IgG). This binding prolongs the half-life of IgG by preventing its lysosomal degradation. The molecular mass of the FCGRT protein is roughly 52 kDa. It is prominently expressed in epithelial and endothelial cells across several tissues including the placenta and liver ensuring IgG stability in circulation.
Biological function summary

FCGRT/FCRN is involved in the transportation and recycling of IgG antibodies. It forms a complex with beta-2 microglobulin that contributes to its function. This complex allows IgG antibodies to traverse cellular barriers such as epithelial cells without being digested. This protein is important in maintaining humoral immunity by regulating IgG levels in the bloodstream and similar processes can be observed in mouse FCRN and cynomolgus monkey milk indicating a conserved mechanism across species.

Pathways

FCGRT/FCRN plays a part in the neonatal Fc receptor pathway. This pathway influences IgG homeostasis and impacts antigen presentation to T cells. By doing so it affects immune surveillance and immune tolerance mechanisms. The pathway involves interactions with related proteins such as albumin which also binds to FCRN for extending its half-life in serum.

Disruptions in FCGRT/FCRN functionality relate to autoimmune conditions and inflammatory diseases. Conditions such as lupus and myasthenia gravis show altered IgG recycling or transportation implicating FCRN in their pathology. Accordingly researchers have explored FCRN inhibitors as potential therapeutic interventions. These inhibitors aim to modulate the interaction between FCRN and IgG therefore impacting the disease progression linked to these disorders.
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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

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Abcam Product Promise

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详情请参阅我们的条款与条件。

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